Optochemical dissection of T-box gene-dependent medial floor plate development

ACS Chemical Biology
Alexander Y PayumoJames K Chen

Abstract

In addition to their cell-autonomous roles in mesoderm development, the zebrafish T-box transcription factors no tail a (ntla) and spadetail (spt/tbx16) are required for medial floor plate (MFP) formation. Posterior MFP cells are completely absent in zebrafish embryos lacking both Ntla and Spt function, and genetic mosaic analyses have shown that the two T-box genes promote MFP development in a non-cell-autonomous manner. On the basis of these observations, it has been proposed that Ntla/Spt-dependent mesoderm-derived signals are required for the induction of posterior but not anterior MFP cells. To investigate the mechanisms by which Ntla and Spt regulate MFP development, we have used photoactivatable caged morpholinos (cMOs) to silence these T-box genes with spatiotemporal control. We find that posterior MFP formation requires Ntla or Spt activity during early gastrulation, specifically in lateral margin-derived cells that converge toward the midline during epiboly and somitogenesis. Nodal signaling-dependent MFP specification is maintained in the absence of Ntla and Spt function; however, midline cells in ntla;spt morphants exhibit aberrant morphogenetic movements, resulting in their anterior mislocalization. Our findings in...Continue Reading

References

Mar 1, 1989·The Journal of Experimental Zoology·G C SchoenwolfL Vakaet
Dec 1, 1993·Neuron·K B Artinger, M Bronner-Fraser
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Jul 15, 1997·Developmental Biology·M E HalpernC B Kimmel
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·M R RebagliatiI B Dawid
Jul 31, 2001·Genesis : the Journal of Genetics and Development·L A EtheridgeM E Halpern
Dec 19, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T DickmeisU Strähle
Jul 10, 2002·Nature Cell Biology·Jason R JessenLilianna Solnica-Krezel
Aug 15, 2002·Trends in Genetics : TIG·Dina C MyersLilianna Solnica-Krezel
Jan 1, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Stéphanie AlbertUwe Strähle
Aug 15, 2003·Development·Iain PattenMarysia Placzek
Mar 2, 2005·Nature Reviews. Neuroscience·Marysia Placzek, James Briscoe
Dec 13, 2006·Developmental Biology·Raymond ErtzerUwe Strähle
Feb 20, 2007·Developmental Biology·James T BennettAlexander F Schier
Aug 25, 2007·Nature Chemical Biology·Ilya A ShestopalovJames K Chen
Jan 15, 2008·Nature Protocols·Christine Thisse, Bernard Thisse
Feb 13, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·P ThévenazM Unser
Jan 31, 2012·Nature Chemical Biology·Ilya A ShestopalovJames K Chen
Jun 13, 2012·Angewandte Chemie·Sayumi YamazoeJames K Chen
Sep 7, 2013·Developmental Biology·Rachel M WargaDonald A Kane
Aug 19, 2014·Angewandte Chemie·Sayumi YamazoeJames K Chen

❮ Previous
Next ❯

Citations

Jul 5, 2016·Nature Chemical Biology·Alexander Y PayumoJames K Chen
May 18, 2017·Nature Chemical Biology·Lukasz Kowalik, James K Chen
Jul 12, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Zachary T MorrowSharon L Amacher

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aphasia

Aphasia affects the ability to process language, including formulation and comprehension of language and speech, as well as the ability to read or write. Here is the latest research on aphasia.