Optofluidic control of rodent learning using cloaked caged glutamate

Proceedings of the National Academy of Sciences of the United States of America
Romain Durand-de CuttoliGraham C R Ellis-Davies

Abstract

Glutamate is the major excitatory neurotransmitter in the brain, and photochemical release of glutamate (or uncaging) is a chemical technique widely used by biologists to interrogate its physiology. A basic prerequisite of these optical probes is bio-inertness before photolysis. However, all caged glutamates are known to have strong antagonism toward receptors of γ-aminobutyric acid, the major inhibitory transmitter. We have developed a caged glutamate probe that is inert toward these receptors at concentrations that are effective for photolysis with violet light. Pharmacological tests in vitro revealed that attachment of a fifth-generation (G5) dendrimer (i.e., cloaking) to the widely used 4-methoxy-7-nitro-indolinyl(MNI)-Glu probe prevented such off-target effects while not changing the photochemical properties of MNI-Glu significantly. G5-MNI-Glu was used with optofluidic delivery to stimulate dopamine neurons of the ventral tegmental area of freely moving mice in a conditioned place-preference protocol so as to mediate Pavlovian conditioning.

References

Feb 1, 1989·Trends in Neurosciences·J H Kaplan, A P Somlyo
Apr 1, 1987·Physiological Reviews·A M Gurney, H A Lester
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E M Callaway, L C Katz
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·R WieboldtG P Hess
Jan 1, 1993·Annual Review of Physiology·S R Adams, R Y Tsien
Apr 25, 1997·Journal of Neuroscience Methods·D W GodwinQ Zhou
Jul 20, 2005·Chemical Communications : Chem Comm·Olesya D FedoryakGraham C R Ellis-Davies
Jul 11, 2006·Angewandte Chemie·Günter Mayer, Alexander Heckel
Jun 22, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Graham C R Ellis-DaviesDwight E Bergles
Apr 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Larry S ZweifelRichard D Palmiter
Jun 10, 2009·Frontiers in Neural Circuits·Elodie FinoRafael Yuste
Jun 19, 2009·Chemical Reviews·Scott H Medina, Mohamed E H El-Sayed
Feb 23, 2010·Nature Chemical Biology·Masanori MatsuzakiGraham C R Ellis-Davies
Mar 5, 2011·Nature Protocols·Graham C R Ellis-Davies
Aug 18, 2010·ACS Chemical Neuroscience·Sarah E CroweGraham C R Ellis-Davies
Jul 26, 2012·Angewandte Chemie·Clara BriekeAlexander Heckel
Apr 13, 2013·Journal of the American Chemical Society·Jeremy P OlsonGraham C R Ellis-Davies
Dec 7, 2013·ACS Chemical Neuroscience·Joseph M AmatrudoGraham C R Ellis-Davies
Jan 25, 2014·Journal of the American Chemical Society·Willem A VelemaBen L Feringa
Dec 5, 2014·The European Journal of Neuroscience·Joseph M AmatrudoGraham C R Ellis-Davies
Jun 24, 2015·Accounts of Chemical Research·Johannes BroichhagenDirk Trauner
Apr 7, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·John E T CorrieDavid R Trentham
Jul 5, 2016·Angewandte Chemie·Michael M LerchBen L Feringa
Dec 3, 2016·Angewandte Chemie·Matthew T RichersGraham C R Ellis-Davies
Feb 22, 2017·Nature Neuroscience·Seongjun ParkPolina Anikeeva
Oct 21, 2017·Journal of Neuroscience Methods·Stefan Passlick, Graham C R Ellis-Davies
Nov 9, 2017·PloS One·Stefan PasslickGraham C R Ellis-Davies
Jul 10, 2018·Chemical Reviews·Katharina HüllDirk Trauner
Nov 15, 2018·Chemical Reviews·Ernst BambergDirk Trauner
Jan 29, 2019·Frontiers in Synaptic Neuroscience·Graham C R Ellis-Davies
Jul 11, 2019·Nature Reviews. Neuroscience·Pierre PaolettiAlexandre Mourot

❮ Previous
Next ❯

Citations

Aug 7, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·E Abou NakadA Specht
Feb 6, 2021·Proceedings of the National Academy of Sciences of the United States of America·Hironori TakahashiYasuteru Urano
Jun 5, 2021·Organic & Biomolecular Chemistry·Davide DeodatoTimothy M Dore
Jun 29, 2021·Médecine sciences : M/S·Romain Durand-de Cuttoli, Alexandre Mourot
Jul 22, 2020·Accounts of Chemical Research·Graham C R Ellis-Davies
Oct 28, 2020·ACS Chemical Neuroscience·James A FrankPolina Anikeeva

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.