Optofluidic differential spectroscopy for absorbance detection of sub-nanolitre liquid samples

Lab on a Chip
Wuzhou Song, Jianhang Yang

Abstract

We present a novel optofluidic differential method for carrying out absorbance spectroscopy of sub-nanolitre volumes of liquid samples on a microfluidic chip. Due to the reduction of liquid volume, the absorbance detection in microfluidics is often hindered by either low sensitivity or complex fabrication. To address this issue, we introduced an optofluidic modulator which can be easily integrated into a PDMS (polydimethylsiloxane) based microfluidic chip. The modulator was controlled by the fluid pressure and the absorbance spectrum of the analyte was obtained by taking differential measurements between the analyte and reference medium. An advantage is that this method doesn't need a complicated fabrication step. It is compatible with conventional microfluidic chips and measurements can be carried out on a normal transmission microscope. The performance of the device was tested by measuring solutions containing methylene blue, with concentrations as low as 13 μM.

References

Jul 28, 2006·Nature·George M Whitesides
Jul 28, 2006·Nature·Paul YagerBernhard H Weigl
Aug 24, 2007·Lab on a Chip·Stefan Haeberle, Roland Zengerle
Oct 26, 2007·Lab on a Chip·Kevin S LeeRajeev J Ram
Feb 12, 2009·Lab on a Chip·Christian H J SchmitzDavid A Weitz
Apr 8, 2009·Lab on a Chip·Michael Rosenauer, Michiel J Vellekoop
Apr 17, 2009·Lab on a Chip·Chaolong SongAnand Krishna Asundi
May 21, 2010·Lab on a Chip·Andreu LloberaStephanus Büttgenbach
Nov 3, 2010·Optics Letters·Wuzhou Song, Demetri Psaltis
Nov 11, 2010·Lab on a Chip·Hongying ZhuAydogan Ozcan
Mar 31, 2011·Lab on a Chip·S XiongY Yang
May 28, 2011·Lab on a Chip·Wuzhou Song, Demetri Psaltis
Jul 9, 2011·Lab on a Chip·Kazuma MawatariTakehiko Kitamori
Jul 30, 2011·Biomicrofluidics·Junji Fukuda, Kohji Nakazawa
Jul 30, 2011·Lab on a Chip·Peng FeiYanyi Huang

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Citations

Aug 7, 2012·Lab on a Chip·Wuzhou SongDemetri Psaltis
Aug 14, 2012·Lab on a Chip·Y C SeowH P Lee
Aug 17, 2012·Lab on a Chip·Cheemeng TanChao-Min Cheng
Jan 15, 2015·Lab on a Chip·Xiaodong HeYouquan Deng
Dec 9, 2017·Biotechnology Journal·Xu-Dong ZhuYong-Hong Wang
May 20, 2021·Analytical Chemistry·Julie ProbstAndrew deMello
Jul 17, 2021·Applied Spectroscopy·Nathan A GiauqueSteven R Goates

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