Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship

Nature Methods
Hidehiko K InagakiDavid J Anderson

Abstract

Optogenetics allows the manipulation of neural activity in freely moving animals with millisecond precision, but its application in Drosophila melanogaster has been limited. Here we show that a recently described red activatable channelrhodopsin (ReaChR) permits control of complex behavior in freely moving adult flies, at wavelengths that are not thought to interfere with normal visual function. This tool affords the opportunity to control neural activity over a broad dynamic range of stimulation intensities. Using time-resolved activation, we show that the neural control of male courtship song can be separated into (i) probabilistic, persistent and (ii) deterministic, command-like components. The former, but not the latter, neurons are subject to functional modulation by social experience, which supports the idea that they constitute a locus of state-dependent influence. This separation is not evident using thermogenetic tools, a result underscoring the importance of temporally precise control of neuronal activation in the functional dissection of neural circuits in Drosophila.

References

Jun 20, 2002·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·D G Stavenga
Sep 2, 1955·Science·E S HODGSONK D ROEDER
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Georg NagelErnst Bamberg
Jun 7, 2005·Cell·Petra StockingerBarry J Dickson
Aug 24, 2005·Nature Neuroscience·Edward S BoydenKarl Deisseroth
Feb 13, 2007·Nature Methods·Mamie Z Li, Stephen J Elledge
May 12, 2007·Current Biology : CB·Greg S B SuhDavid J Anderson
Mar 18, 2008·Neuron·Liqun LuoKarel Svoboda
Jun 13, 2008·Nature·Fumika N HamadaPaul A Garrity
Jul 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Barret D PfeifferGerald M Rubin
Dec 17, 2008·Nature Neuroscience·André BerndtKarl Deisseroth
Feb 17, 2009·Neuron·Michael D Gordon, Kristin Scott
Mar 10, 2009·Nature Methods·Heiko DankertPietro Perona
Oct 17, 2009·IEEE Transactions on Visualization and Computer Graphics·Yong WanCharles Hansen
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Satoko YamaguchiMartin Heisenberg
Jun 26, 2010·Frontiers in Behavioral Neuroscience·Dennis BellmannKlemens F Störtkuhl
Aug 11, 2010·Genetics·Barret D PfeifferGerald M Rubin
Sep 14, 2010·Current Biology : CB·Jai Y YuBarry J Dickson
Feb 15, 2011·Neuron·Anne C von PhilipsbornBarry J Dickson
Jun 23, 2011·Annual Review of Neuroscience·Lief FennoKarl Deisseroth
Jul 13, 2011·Neuron·Ofer YizharKarl Deisseroth
Feb 9, 2012·Journal of Computational Neuroscience·Daniela BianchiMichele Migliore
May 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yufeng PanBruce S Baker
Jul 24, 2012·Neuroscience·Alex C Keene, Pavel Masek
Oct 16, 2012·Cell Reports·Arnim JenettChristopher T Zugates
Dec 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Timothy D TaylerDavid J Anderson

❮ Previous
Next ❯

Citations

Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ming-Chin WuAnn-Shyn Chiang
May 27, 2014·Nature Methods·Daniel E BathAndrew D Straw
Sep 8, 2015·ELife·Stefanie HampelAndrew M Seeds
May 1, 2015·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Lovesha Sivanantharajah, Bing Zhang
Aug 5, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David OwaldScott Waddell
Jan 8, 2015·Frontiers in Neuroengineering·Joshua I Sanders, Adam Kepecs
May 18, 2016·Current Opinion in Neurobiology·Eric D Hoopfer
Jun 14, 2016·Neuron·Stephen X ZhangMichael A Crickmore
Nov 1, 2016·Nature Neuroscience·Junjie LuoCraig Montell
Oct 19, 2016·Nature Reviews. Neuroscience·David J Anderson
Jan 6, 2017·Scientific Reports·Serena BovettiTommaso Fellin
Jul 2, 2015·Scientific Reports·Ulrich SternChung-Hui Yang
Aug 28, 2014·PloS One·Rod S LimDavid J Anderson
Oct 4, 2014·Neuron·David J Anderson, Pietro Perona
Mar 30, 2017·Biophysical Journal·Benjamin S KrausePeter Hegemann
Nov 29, 2017·Nature Communications·Melvyn H W YapBruno van Swinderen
Dec 20, 2017·Scientific Reports·Ema Suzuki-SawanoMinoru Saitoe
Nov 10, 2017·Journal of Neurogenetics·Fernando Martín, Esther Alcorta
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Fang GuoMichael Rosbash
Jul 12, 2018·Nature·Laura F SeeholzerVanessa Ruta
Feb 11, 2014·Nature Methods·Nathan C KlapoetkeEdward S Boyden
Jun 18, 2014·Nature Chemical Biology·Arnaud GautierSophie Vriz
Jul 17, 2015·Nature Communications·Hsiang-Wen ShihAnn-Shyn Chiang
Jul 1, 2015·Nature Communications·Shu-Yun KuoTsai-Feng Fu
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Alexej DawydowRobert J Kittel
Aug 21, 2019·Pest Management Science·Hao GuoDean Smith
Jul 19, 2019·The Journal of Experimental Biology·Sweta Agrawal, Michael H Dickinson
Sep 13, 2019·The Journal of Experimental Biology·Yu-Chieh David ChenAnupama Dahanukar
Jan 13, 2018·Annual Review of Entomology·Kristin Scott
Aug 20, 2019·Genes, Brain, and Behavior·Kosei Sato, Daisuke Yamamoto
Dec 8, 2019·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Karen Y Cheng, Mark A Frye
Jan 8, 2020·Journal of Neurogenetics·Nadine Ehmann, Dennis Pauls

❮ Previous
Next ❯

Methods Mentioned

BETA
dissection
transgenic
PCR

Software Mentioned

Clampex
imageJ
ReaChR
Fluorender
Clampfit
Matlab

Related Concepts

Related Feeds

BRAIN Initiative Cell Census Network (BICCN)

The BRAIN Initiative Cell Census Network aims to identify and provide experimental access to the different brain cell types to determine their roles in health and disease. Discover the latest research from researchers in the BRAIN Initiative Cell Census Network here.