Optogenetic reporters: Fluorescent protein-based genetically encoded indicators of signaling and metabolism in the brain.

Progress in Brain Research
Mathew TantamaGary Yellen

Abstract

Fluorescent protein technology has evolved to include genetically encoded biosensors that can monitor levels of ions, metabolites, and enzyme activities as well as protein conformation and even membrane voltage. They are well suited to live-cell microscopy and quantitative analysis, and they can be used in multiple imaging modes, including one- or two-photon fluorescence intensity or lifetime microscopy. Although not nearly complete, there now exists a substantial set of genetically encoded reporters that can be used to monitor many aspects of neuronal and glial biology, and these biosensors can be used to visualize synaptic transmission and activity-dependent signaling in vitro and in vivo. In this review, we present an overview of design strategies for engineering biosensors, including sensor designs using circularly permuted fluorescent proteins and using fluorescence resonance energy transfer between fluorescent proteins. We also provide examples of indicators that sense small ions (e.g., pH, chloride, zinc), metabolites (e.g., glutamate, glucose, ATP, cAMP, lipid metabolites), signaling pathways (e.g., G protein-coupled receptors, Rho GTPases), enzyme activities (e.g., protein kinase A, caspases), and reactive species. We ...Continue Reading

Citations

May 21, 2013·Annual Review of Neuroscience·Z Josh Huang, Hongkui Zeng
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Mark JuhasNenad Bursac
Feb 16, 2013·Biochimica Et Biophysica Acta·Jennifer R Shell, David S Lawrence
Apr 1, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Hideyuki OkanoKiyoto Kasai
Feb 25, 2015·Nanomedicine·Dusica Maysinger, Eliza Hutter
Nov 5, 2014·Frontiers in Neural Circuits·Dona K MurpheyBenjamin R Arenkiel
Jun 18, 2016·Science·Xiaolu A CambronneRichard H Goodman
Dec 13, 2016·Free Radical Biology & Medicine·Dmitry S Bilan, Vsevolod V Belousov
Nov 28, 2015·Viruses·Ian B HogueLynn W Enquist
Dec 19, 2017·Chemosensors·Zhijie ChenHui-Wang Ai
Sep 30, 2014·Journal of Bioenergetics and Biomembranes·Niamh M C Connolly, Jochen H M Prehn
Jun 27, 2014·Epilepsia·Elsa RossignolAlbert J Becker
Mar 24, 2015·Nature Chemical Biology·Lionel TarragoVadim N Gladyshev
Mar 25, 2015·Journal of Neuroscience Research·James B HurleyJianhai Du
Feb 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yuhong CaoNicholas A Melosh
Dec 13, 2017·Cell Death and Differentiation·Niamh M C ConnollyJochen H M Prehn
May 7, 2020·Frontiers in Cellular Neuroscience·Rodrigo LerchundiChristine R Rose
May 24, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Joshua R St ClairCatherine Proenza
Dec 7, 2018·Journal of Neuroscience Research·Rodrigo LerchundiChristine R Rose
Jul 31, 2019·Translational Research : the Journal of Laboratory and Clinical Medicine·Karen ScidaBrian G Jamieson
Nov 11, 2020·Trends in Neurosciences·David M Lovinger, Christina M Gremel
Nov 3, 2017·Neuron·Benjamin R RostPeter Hegemann

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