Optogenetic stimulation of Drosophila heart rate at different temperatures and Ca2+ concentrations

Physiological Reports
Yue C ZhuRobin L Cooper

Abstract

Optogenetics is a revolutionary technique that enables noninvasive activation of electrically excitable cells. In mammals, heart rate has traditionally been modulated with pharmacological agents or direct stimulation of cardiac tissue with electrodes. However, implanted wires have been known to cause physical damage and damage from electrical currents. Here, we describe a proof of concept to optically drive cardiac function in a model organism, Drosophila melanogaster. We expressed the light sensitive channelrhodopsin protein ChR2.XXL in larval Drosophila hearts and examined light-induced activation of cardiac tissue. After demonstrating optical stimulation of larval heart rate, the approach was tested at low temperature and low calcium levels to simulate mammalian heart transplant conditions. Optical activation of ChR2.XXL substantially increased heart rate in all conditions. We have developed a system that can be instrumental in characterizing the physiology of optogenetically controlled cardiac function with an intact heart.

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Citations

Dec 19, 2017·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Chan JiangZi Qiang Liu
Mar 4, 2017·The Journal of Experimental Biology·Kay LammersAchim Paululat
Aug 25, 2019·Methods and Protocols·Catherine E StanleyRobin L Cooper
Jun 1, 2020·Invertebrate Neuroscience : in·Catherine E StanleyRobin L Cooper
Jun 15, 2017·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Cole MalloyRobin L Cooper
May 23, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Yue Chen ZhuRobin L Cooper
Jul 1, 2020·Communications Biology·Jing MenChao Zhou
Aug 22, 2018·Scientific Reports·Darío BalcazarPaola Ferrero
Nov 15, 2016·Clinical Medicine Insights. Cardiology·Thomas V KarathanosNatalia A Trayanova
Dec 20, 2020·Nature Reviews. Cardiology·Emilia Entcheva, Matthew W Kay
Jul 8, 2021·Cellular and Molecular Life Sciences : CMLS·Wen Kin LimNicholas S Tolwinski

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