Optoregulated force application to cellular receptors using molecular motors.

Nature Communications
Yijun ZhengAránzazu Del Campo

Abstract

Progress in our understanding of mechanotransduction events requires noninvasive methods for the manipulation of forces at molecular scale in physiological environments. Inspired by cellular mechanisms for force application (i.e. motor proteins pulling on cytoskeletal fibers), we present a unique molecular machine that can apply forces at cell-matrix and cell-cell junctions using light as an energy source. The key actuator is a light-driven rotatory molecular motor linked to polymer chains, which is intercalated between a membrane receptor and an engineered biointerface. The light-driven actuation of the molecular motor is converted in mechanical twisting of the entangled polymer chains, which will in turn effectively "pull" on engaged cell membrane receptors (e.g., integrins, T cell receptors) within the illuminated area. Applied forces have physiologically-relevant magnitude and occur at time scales within the relevant ranges for mechanotransduction at cell-friendly exposure conditions, as demonstrated in force-dependent focal adhesion maturation and T cell activation experiments. Our results reveal the potential of nanomotors for the manipulation of living cells at the molecular scale and demonstrate a functionality which at...Continue Reading

References

Sep 18, 1999·Nature·N KoumuraB L Feringa
Apr 18, 2003·Nature·Manfred Schliwa, Günther Woehlke
Aug 25, 2006·The Journal of Physical Chemistry. B·Philip C NelsonDavid Dunlap
Sep 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Nathan J SniadeckiChristopher S Chen
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Diana E Jaalouk, Jan Lammerding
Sep 17, 2009·The Journal of Biological Chemistry·Sun Taek KimEllis L Reinherz
Nov 1, 2011·Nature Methods·Daniel R StableyKhalid S Salaita
Feb 22, 2012·Biophysical Journal·Edward JudokusumoLance C Kam
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Mar 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Pere Roca-CusachsMichael P Sheetz
Jan 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Keenan T BashourLance C Kam
Oct 31, 2014·Nature Reviews. Molecular Cell Biology·Jay D HumphreyMartin A Schwartz
Mar 18, 2016·Nature Communications·Darren YangWesley P Wong
Apr 29, 2016·Nature Methods·William J Polacheck, Christopher S Chen
Dec 3, 2016·Angewandte Chemie·Laith F KademChristine Selhuber-Unkel
Mar 21, 2017·Nature Nanotechnology·Justin T FoyNicolas Giuseppone
Sep 1, 2017·Nature·Víctor García-LópezJames M Tour
May 2, 2018·Proceedings of the National Academy of Sciences of the United States of America·Diederik RokeBen L Feringa
Jan 4, 2019·Nature Cell Biology·Zhiqi SunReinhard Fässler
Mar 13, 2019·Nanoscale·Jean-Rémy Colard-IttéNicolas Giuseppone
Aug 16, 2019·Chemical Reviews·Massimo BaronciniAlberto Credi
Dec 24, 2019·Chemical Reviews·Damien DattlerNicolas Giuseppone

❮ Previous
Next ❯

Software Mentioned

ImageJ
MATLAB
Particle Tracker
Morpholib
SysCon

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.