Oral administration of plant-based rotavirus VP6 induces antigen-specific IgAs, IgGs and passive protection in mice

Vaccine
Bo ZhouDawei Li

Abstract

Plant viruses show significant potential as expression vectors for producing foreign proteins in plants. In this study, codon-optimized VP6 gene (sVP6) of human rotavirus was engineered as a replacement to the coat protein (CP) open reading frame of Beet black scorch virus (BBSV). In vitro-generated RNA transcripts corresponding to the engineered virus were infectious when inoculated onto the leaves of Chenopodium amaranticolor. Molecular analysis revealed that sVP6 was efficiently expressed and accounted for 0.25% of the total soluble protein (TSP) in plant leaves on 7 dpi. On average, a high level 1.54 microg of sVP6 was expressed in each gram of infected leaves. Oral immunization of female BALB/c mice with the plant-based sVP6 protein induced high titers of anti-VP6 mucosal IgA and serum IgG. 60% of suckling pups born from immunized dams were protected against the virulent rotavirus challenge and those infected pups developed less severe diarrhea. These results suggested that it is feasible to induce lactogenic immunity against an enteric pathogen through oral vaccination, by using the antigen produced in a new BBSV-based plant protein expression system.

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Citations

Aug 28, 2014·Expert Review of Vaccines·Marisela HernándezEdda Sciutto
Nov 17, 2011·Vaccine·Vincent PavotStéphane Paul
Aug 8, 2015·Plant Biotechnology Journal·Vanesa RuizAndrés Wigdorovitz
Dec 3, 2015·Virology Journal·Francisco F P G PêraInga I Hitzeroth
Apr 8, 2014·Research in Veterinary Science·Mia Madel AlfajaroKyoung-Oh Cho
Sep 3, 2013·Vaccine·Hajnalka PappKrisztián Bányai
May 30, 2015·Vaccine·Somayeh JalilvandZabihollah Shoja
Mar 29, 2011·Vaccine·Nicolas RochereauStéphane Paul
Jul 22, 2016·Plant Biotechnology Journal·Naila Shahid, Henry Daniell
Oct 19, 2016·International Journal of Molecular Sciences·Young Hee JoungHyun-Soon Kim
Jan 8, 2019·Reviews in Medical Virology·Atefeh AfchangiZabihollah Shoja

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