Oral administration of recombinant human granulocyte-macrophage colony stimulating factor expressed in rice endosperm can increase leukocytes in mice

Biotechnology Letters
Tingting NingDaichang Yang

Abstract

Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is used clinically to treat leucopenia typically caused by cancer chemotherapy or radiotherapy. This study used multiple strategies to obtain very high expression levels of OsrhGM-CSF (14 microg/seed) in rice endosperm. Electron micrographs of immunogold-labeled transgenic endosperm showed that rhGM-CSF was not only localized in protein bodies but was also distributed in the apoplast. A biological activity assay indicated that OsrhGM-CSF stimulated the growth of TF-1 cells in vitro. In addition, the transgene was used to effectively treat leucopenia by oral administration of the unprocessed transgenic grains. In cyclophosphamide-induced leucopenic mice, transgenic seeds produced a 27% (t=0.021) gain in leukocytes after 14 days feeding. Even in non-leucopenic mice, leukocyte gain was 37% (t=0.002) more than that of mice fed non-transgenic seeds. This study provides a novel approach to the use of oral unprocessed transgenic OsrhGM-CSF seeds to treat leucopenia.

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Citations

Nov 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yang HeDaichang Yang
Dec 30, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Anna Góra-SochackaAgnieszka Sirko
Jul 13, 2011·International Journal of Molecular Sciences·Agnieszka SirkoPatrycja Redkiewicz
Feb 26, 2013·International Journal of Molecular Sciences·Na AnDaichang Yang
Jan 15, 2014·Plant Cell Reports·Jiquan OuDaichnag Yang
Feb 5, 2013·Journal of Biotechnology·Liping ZhangDaichang Yang
Jan 1, 2016·Applied Biochemistry and Microbiology·M S BurlakovskiyL A Lutova
Aug 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Evangelia VamvakaTeresa Capell
Jul 4, 2020·Journal of Biotechnology·Jianlei PangDaichang Yang
Sep 10, 2008·Journal of Proteome Research·Junling LuoDaichang Yang

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