Oral and intraperitoneal administration of phosphorothioate oligodeoxynucleotides leads to control of Cryptosporidium parvum infection in neonatal mice

The Journal of Infectious Diseases
Mathieu BarrierFabrice Laurent

Abstract

Neonates are particularly vulnerable to infections, in part because of the incomplete development of their immune system. Recent advances in immunostimulatory treatments based on conserved microbial components led us to assess the potential of oligodeoxynucleotides (ODNs) for decreasing the sensitivity of neonates to Cryptosporidium parvum infection. Neonate mice were treated orally or intraperitoneally (ip) with CpG ODNs or non-CpG ODNs 24 h before C. parvum infection, and parasite load and cytokine up-regulation were evaluated. CpG ODN 1668 and non-CpG ODN 1668 administered orally, as well as CpG ODN 1668 administered ip, induced an 80%-95% decrease in intestinal parasite load 6 days after infection. Intraperitoneal and oral pretreatment with CpG ODN 1668 led to a strong initial up-regulation of cytokines and CD69 messenger RNA in the intestine and a decrease in parasite load by a Toll-like receptor 9 (TLR9)-dependent mechanism. By contrast, oral administration of non-CpG ODN 1668 decreased parasite load by a TLR9-independent mechanism. The control of neonatal C. parvum infection by ip or oral administration of ODNs is feasible by 2 different mechanisms: (1) the well-known interaction involving CpG/TLR9, leading to the produc...Continue Reading

Citations

Apr 26, 2007·Nature Reviews. Immunology·Ofer Levy
Sep 16, 2011·Clinical and Vaccine Immunology : CVI·Patricio A ManqueGregory A Buck
Jun 3, 2010·Infection and Immunity·Andrea EcheverryBecky Adkins
Feb 28, 2007·Infection and Immunity·Andrea EcheverryBecky Adkins
Dec 17, 2009·PloS One·Sonia Lacroix-LamandéFabrice Laurent
Jul 4, 2007·Proceedings of the American Thoracic Society·Arthur M Krieg
Jun 30, 2011·The Journal of Parasitology·Lourrany B CostaCirle Alcantara Warren
Jun 3, 2008·Developmental and Comparative Immunology·Isabel Tourais-EstevesFabrice Laurent
Nov 24, 2012·Parasite Immunology·V McDonaldF Petry
Sep 10, 2014·Parasite Immunology·C L Wilhelm, F Yarovinsky
Nov 17, 2009·Cellular Microbiology·Peter LiehlMaria M Mota
Mar 19, 2014·Human Vaccines & Immunotherapeutics·Jan R Mead
Jul 28, 2016·Parasite Immunology·U RyanA Paparini
Jun 2, 2016·Infection and Immunity·Cynthia L ChappellJames Hixson
Sep 12, 2018·The Journal of Infectious Diseases·Laurent PotironFabrice Laurent
Oct 28, 2011·Expert Review of Anti-infective Therapy·Vincent McDonald
Mar 9, 2012·Expert Review of Anti-infective Therapy·Eliana Akemi FutataMaria Notomi Sato
Nov 7, 2019·Science Translational Medicine·Christopher SwaleMohamed-Ali Hakimi
Aug 19, 2015·Current Tropical Medicine Reports·Jacob G Ludington, Honorine D Ward
Jan 2, 2018·Archives of Medical Research·Oswaldo Partida-RodríguezCecilia Ximenez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.