Oral bacterial extracts facilitate early osteogenic/dentinogenic differentiation in human dental pulp-derived cells

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Shu AbeMasaki J Honda

Abstract

Bacterial metabolites demineralize dental hard tissues, and soluble factors lead to tertiary dentinogenesis in the area of the dentin-pulp complex. However, it is unclear whether the oral bacteria are directly involved in the differentiation of dental pulp cells. In this study, we evaluated the effect of oral bacterial extracts on cellular differentiation in human dental pulp-derived cells (hDPC). The hDPC were obtained from third molar teeth, and the cells were subcultured. The sonicated extracts were obtained from Porphyromonas gingivalis (gram-negative) and Streptococcus mutans (gram-positive). The effect of bacterial extracts on cellular growth and differentiation in hDPC were tested. Alkaline phosphatase activity and bone sialoprotein (BSP) gene expression were increased in hDPC exposed to low concentrations of both sonicated extracts, whereas the activity was decreased upon exposure to high concentrations of sonicated extracts from P. gingivalis. This is the first evidence that oral bacteria have a positive effect on cellular differentiation in hPDC.

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Citations

Jun 11, 2015·Journal of Cellular and Molecular Medicine·Kyriaki ChatzivasileiouHermann Lang
Jun 26, 2017·Stem Cell Research & Therapy·Ruili YangYanheng Zhou
May 24, 2011·Journal of Materials Science. Materials in Medicine·Sandra Viale-BouroncleChristian Morsczeck
Dec 10, 2020·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Panuroot AguilarVeera Lertchirakarn
Sep 21, 2020·Journal of Endodontics·Nastaran MeschiNikita B Ruparel
Nov 28, 2018·Journal of Endodontics·Obadah AustahNikita B Ruparel

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