Oral cancer detection using diffuse reflectance spectral ratio R540/R575 of oxygenated hemoglobin bands

Journal of Biomedical Optics
Narayanan SubhashJayaprakash Madhavan

Abstract

A low-cost, fast, and noninvasive method for early diagnosis of malignant lesions of oral mucosa based on diffuse reflectance spectral signatures is presented. In this technique, output of a tungsten halogen lamp is guided to the tissue through the central fiber of a reflection probe whose surrounding six fibers collects tissue reflectance. Ex vivo diffuse reflectance spectra in the 400 to 600-nm region is measured from surgically removed oral cavity lesions using a miniature fiber optic spectrometer connected to a computer. Reflectance spectral intensity is higher in malignant tissues and shows dips at 542 and 577 nm owing to absorption from oxygenated hemoglobin (HbO2). Measurements carried out, within an hour of surgical excision, on malignant lesion and adjoining uninvolved mucosa show that these absorption features are more prominent in neoplastic tissues owing to increased microvasculature and blood content. It is observed that reflectance intensity ratio of hemoglobin bands, R540/R575, from malignant sites are always lower than that from normal sites and vary according to the histological grade of malignancy. The diffuse reflectance intensity ratio R540/R575 of the hemoglobin bands appears to be a useful tool to discrimi...Continue Reading

References

Jan 1, 1994·CA: a Cancer Journal for Clinicians·C C BoringS Montgomery
Jan 1, 1995·Lasers in Surgery and Medicine·J R MourantT Shimada
Jun 30, 2001·Lasers in Surgery and Medicine·U UtzingerR Richards-Kortum
Mar 14, 2002·Lasers in Surgery and Medicine·Gregory M PalmerNirmala Ramanujam
Dec 11, 2007·Optics Letters·Q ZhangM S Feld
Jan 1, 1996·Journal of Biomedical Optics·A Mahadevan-Jansen, R R Richards-Kortum

❮ Previous
Next ❯

Citations

Apr 3, 2012·Journal of Biomedical Optics·Prasanth Chandra SekharNarayanan Subhash
May 10, 2008·Journal of Biomedical Optics·Darren RoblyerAnn M Gillenwater
Jan 7, 2009·Journal of Biomedical Optics·Sasha McGeeGregory Grillone
Nov 2, 2015·Lasers in Medical Science·Vasumathi Gopala PrabithaNarayanan Subhash
Jan 22, 2011·Clinical Oral Implants Research·Ralph van BrakelMarco S Cune
Oct 15, 2009·Head & Neck·Rupananda Jayachandra MalliaJayaprakash Madhavan
Apr 28, 2016·The Journal of the American Dental Association·Yastira LallaCamile S Farah
Apr 24, 2010·Applied Spectroscopy·Rupananda Jayachandra MalliaJayakrishnan Radhakrishnan
Nov 22, 2008·Journal of Biomedical Optics·Christian ZakianDavid Hamlin
Apr 2, 2014·Biomedical Optics Express·Bing YuDaron G Ferris
Jul 4, 2013·Journal of Biophotonics·Hemant KrishnaPradeep Kumar Gupta
Dec 4, 2013·Biomedical Optics Express·Li-Tzu LeeHsing-Wen Wang
Jul 1, 2017·Biomedical Optics Express·David M Miller, Nan M Jokerst
Apr 30, 2020·Minimally Invasive Therapy & Allied Technologies : MITAT : Official Journal of the Society for Minimally Invasive Therapy·Wenwen LiuXuemei Yan
Mar 21, 2018·International Journal of Oral Science·Chenzhou WuEben Rosenthal
Jul 1, 2017·Biomedical Optics Express·Adam ShadfanTomasz S Tkaczyk
Nov 22, 2008·Journal of Biomedical Optics·Rupananda MalliaNarayanan Subhash
Feb 8, 2013·Optics Express·Venkataramanan KrishnaswamyBrian W Pogue
Jun 15, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Guolan LuBaowei Fei
Dec 15, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·S RameshRamapurath S Jayasree
Oct 13, 2009·Analytical Chemistry·Jianhua MoZhiwei Huang
Aug 31, 2021·World Journal of Gastrointestinal Oncology·Toru ChibaTomohiko Akahoshi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.