Oral cancer overexpressed 1 (ORAOV1) regulates cell cycle and apoptosis in cervical cancer HeLa cells.

Molecular Cancer
Lu JiangQianming Chen

Abstract

Oral Cancer Overexpressed 1 (ORAOV1) is a candidate protooncogene locating on 11q13. Recent studies show that ORAOV1 acts as a primary driving force behind 11q13 gene amplification and plays a functional role in the tumorigenesis in a variety of human squamous cell carcinomas (SCCs). According to the results of molecular cytogenetic methods, 11q13 was characterized to be a high-level and recurrent amplification chromosomal site in cervical cancers. Up till now, the role of ORAOV1 in cervical cancer is unknown. The purpose of this study is to elucidate the function of ORAOV1 in cervical cancer cell growth by studying its roles in HeLa cells using small interfering RNA. Functional analyses revealed that ORAOV1 was involved in the regulation of HeLa cell growth through its effect on cell cycle and apoptosis. Silence of ORAOV1 in HeLa cells downregulated the expression of Cyclin A, Cyclin B1 and Cdc2, and led to a distinct S cell cycle arrest. Moreover, knockdown of ORAOV1 expression activated both extrinsic and intrinsic apoptotic pathways and led to apoptosis in HeLa cells through its effect on the expression of several apoptosis related proteins such as P53, Bcl-2, Caspase-3, Caspase-8, Caspase-9 and cytochrome c. Interestingly,...Continue Reading

References

Jul 1, 1992·European Journal of Cancer. Part B, Oral Oncology·J K Field
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·S A InnocenteJ M Lee
Nov 14, 2000·Molecular and Cellular Biology·R A BlakeS A Courtneidge
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Patrick J PaddisonGregory J Hannon
Aug 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xin HuangTony E Godfrey
Sep 16, 2003·Journal of Cell Science·Susan HauptYgal Haupt
Oct 17, 2003·Genes & Development·Jerry M Adams
Dec 14, 2004·Journal of Molecular Endocrinology·I BantounasJ B Uney
Jan 11, 2005·Oncogene·Suqing XieWei Dai
Jun 17, 2006·Cancer Research·Eun-Jin YeoJong-Wan Park
May 17, 2007·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Y-W ChoiW S Ahn
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Jul 17, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Ralph P InsingaMargaret M Madeleine
Aug 9, 2008·International Journal of Cancer. Journal International Du Cancer·Lu JiangQianming Chen
Feb 5, 2009·Molecular and Cellular Biochemistry·Mayank Singh, Neeta Singh
Feb 6, 2009·Journal of Cell Science·Joslyn K Brunelle, Anthony Letai
Jun 6, 2009·Journal of Dental Research·L JiangQ M Chen

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Citations

Jul 2, 2011·Virology Journal·Helen L Del PuertoAnilton C Vasconcelos
Sep 6, 2012·International Journal of Biological Sciences·Anja TorsvikRolf Bjerkvig
Dec 12, 2012·Genes, Chromosomes & Cancer·Paul M Wilkerson, Jorge S Reis-Filho
Nov 11, 2011·Genes, Chromosomes & Cancer·Srikant AmbatipudiManoj B Mahimkar
Oct 10, 2015·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Xin ZhaoQianming Chen
Apr 2, 2013·American Journal of Human Genetics·Juliet D FrenchAlison M Dunning
Jan 19, 2017·International Journal of Molecular Sciences·Yao Qin, Shijun J Zheng
Jun 10, 2016·International Journal of Oncology·Xuefeng ZhangQianming Chen
Nov 28, 2019·Scientific Reports·Ellen WikeniusAnders Berglund
Nov 9, 2020·Journal of Cancer Research and Clinical Oncology·Sang Yun HaSeok-Hyung Kim

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Methods Mentioned

BETA
transfection
PCR
flow cytometry
xenografts
xenograft
flow cytometrer
fluorescence-activated cell sorting

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