Oral colonisation by antimicrobial-resistant Gram-negative bacteria among long-term care facility residents: prevalence, risk factors, and molecular epidemiology.

Antimicrobial Resistance and Infection Control
Mi Nguyen-Tra LeMotoyuki Sugai

Abstract

For residents of long-term care facilities (LTCFs), antimicrobial-resistant bacteria (ARB) are a risk factor, yet their oral colonisation, potentially leading to aspiration pneumonia, remains unclear. This study was undertaken to survey the prevalence, phenotypic characteristics, and molecular epidemiology of antimicrobial-resistant Gram-negative bacteria in the oral cavity of LTCF residents, and to analyse the risk factors for such carriers. This study involved 98 residents of a LTCF in Hiroshima City, Japan, aged between 55 and 101 years. Oropharyngeal swabs were collected and plated on screening media for ESBL-producing and carbapenem-resistant bacteria; isolates were identified and tested for antibiotic susceptibility; biofilm formation was tested in vitro; identification of epidemic clones were pre-determined by PCR; resistance genes, sequence types, and whole-genome comparison of strains were conducted using draft genome sequences. Demographic data and clinical characterisations were collected and risk factors analysed. Fifty-four strains from 38% of the residents grew on screening media and comprised predominantly of Acinetobacter spp. (35%), Enterobacteriaceae spp. (22%), and Pseudomonas spp. (19%). All Escherichia coli...Continue Reading

References

Nov 20, 1969·The New England Journal of Medicine·W G JohansonJ P Sanford
Jan 1, 1996·Clinical Microbiology Reviews·L E NicolleR A Garibaldi
Sep 30, 2000·Applied and Environmental Microbiology·O ClermontE Bingen
Oct 7, 2000·Antimicrobial Agents and Chemotherapy·G S Babini, D M Livermore
Oct 12, 2001·International Journal of Systematic and Evolutionary Microbiology·A NemecL Dijkshoorn
Jul 13, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Scott D HanesTimothy C Fabian
Aug 27, 2002·Emerging Infectious Diseases·Rodney M Donlan
Sep 12, 2003·Emerging Infectious Diseases·Arthur LeibovitzRephael Segal
Aug 13, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Curtis J Donskey
Nov 4, 2004·JAMA : the Journal of the American Medical Association·Thomas M GillZhenchao Guo
Dec 23, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Vincent QuagliarelloMary Tinetti
Apr 16, 2005·Clinical Microbiology Reviews·Timothy R WalshPatrice Nordmann
Jun 9, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·George A Jacoby
Mar 7, 2006·Journal of Clinical Microbiology·Bernard La ScolaDidier Raoult
Mar 21, 2008·The New England Journal of Medicine·L Silvia Munoz-Price, Robert A Weinstein
Jul 16, 2008·Clinical Microbiology Reviews·Anton Y PelegDavid L Paterson
Aug 19, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A MarchN Woodford
Nov 26, 2009·International Journal of Medical Microbiology : IJMM·Krisztina MáderSándor Túri
Feb 11, 2011·Journal of Visualized Experiments : JoVE·George A O'Toole
Aug 10, 2011·BMC Genomics·Nabil-Fareed AlikhanScott A Beatson
Jan 13, 2012·Journal of Clinical Microbiology·Mette V LarsenOle Lund
Jun 27, 2012·The Journal of Antimicrobial Chemotherapy·Shiro EndoMitsuo Kaku
Jul 12, 2012·The Journal of Antimicrobial Chemotherapy·Ea ZankariMette Voldby Larsen
Dec 3, 2013·Nucleic Acids Research·Ross OverbeekRick Stevens
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nicola K PettyScott A Beatson
Dec 23, 2015·Emerging Infectious Diseases·Helmut J F SalzerHarald Seifert
Apr 4, 2017·FEMS Microbiology Reviews·Clayton W Hall, Thien-Fah Mah
May 11, 2017·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E SieswerdaK van Dijk
Jun 7, 2017·Antimicrobial Agents and Chemotherapy·Yasufumi MatsumuraSatoshi Ichiyama
Aug 8, 2017·International Journal of Antimicrobial Agents·Myrto Eleni FlokasEleftherios Mylonakis
Jan 18, 2018·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Naoko KariyaHiroyasu Iso
Sep 19, 2018·New Microbes and New Infections·Cl Soria SegarraCa Soria Segarra

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Citations

May 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tania YacoubJean-Marc Sabatier And Ziad Fajloun
Apr 10, 2021·Nature Reviews. Disease Primers·Antoni TorresTom van der Poll
May 11, 2021·Frontiers in Cellular and Infection Microbiology·Hsin-Yu ChenPo-Ren Hsueh

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Datasets Mentioned

BETA
EC958
CP021454
ATCC17987
AP012280

Methods Mentioned

BETA
PCR

Software Mentioned

MLST
Blast Ring Image Generator ( BRIG )
Rapid Annotation using Subsystem Technology ( RAST )
SPSS
Vitek
ResFinder

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