Oral health status in historic population: Macroscopic and metagenomic evidence

PloS One
Claire WillmannCatherine Thèves

Abstract

Recent developments in High-Throughput DNA sequencing (HTS) technologies and ancient DNA (aDNA) research have opened access to the characterization of the microbial communities within past populations. Most studies have, however, relied on the analysis of dental calculus as one particular material type particularly prone to the molecular preservation of ancient microbial biofilms and potential of entire teeth for microbial characterization, both of healthy communities and pathogens in ancient individuals, remains overlooked. In this study, we used shotgun sequencing to characterize the bacterial composition from historical subjects showing macroscopic evidence of oral pathologies. We first carried out a macroscopic analysis aimed at identifying carious or periodontal diseases in subjects belonging to a French rural population of the 18th century AD. We next examined radiographically six subjects showing specific, characteristic dental pathologies and applied HTS shotgun sequencing to characterize the microbial communities present in and on the dental material. The presence of Streptococcus mutans and also Rothia dentocariosa, Actinomyces viscosus, Porphyromonas gingivalis, Tannerella forsythia, Pseudoramibacter alactolyticus, O...Continue Reading

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Citations

Nov 24, 2019·Scientific Reports·Andrew T OzgaAnne C Stone
May 14, 2020·International Journal of Molecular Sciences·Yingzhi Gu, Xiaozhe Han
Jul 18, 2021·BMC Oral Health·Simone G OliveiraFlavio H B Aguiar

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Datasets Mentioned

BETA
SRR5581851-58
SRR6785785-87

Methods Mentioned

BETA
X-ray
PCR
chips
deamination

Software Mentioned

MALT
IGV
GraphPad Prism
MetaPhlAn

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