Oral immunization of mice with a multivalent therapeutic subunit vaccine protects against Helicobacter pylori infection.

Vaccine
Meiying LiuBo Wei

Abstract

Helicobacter pylori is a human class I carcinogen and no effective prophylactic or therapeutic H. pylori vaccine has yet been marketed. H. pylori can escape the host immune response, but the precise immune protection mechanisms in humans remain unknown. In this study, we developed a multivalent, subunit H. pylori vaccine candidate by formulating three commonly used H. pylori antigens, neutrophil-activating protein (NAP), urease subunit A (UreA) and subunit B (UreB) with the mucosal adjuvant, a double-mutant heat-labile toxin (dmLT) from Escherichia coli, and evaluated its immunogenicity and therapeutic efficacy in a mouse model of H. pylori infection. We found that oral immunization of H. pylori-infected mice significantly reduced gastric bacterial colonization at both 2 and 8 weeks after immunization. The reduction in bacterial burdens was accompanied with significantly increased serum antigen-specific IgG responses and mucosal IgA responses. Moreover, oral immunization also induced Th1/Th17 immune responses, which may play a synergistic role with the specific antibodies in the elimination of H. pylori. Thus, our vaccine candidate appears able to overcome the immune evasion mechanism of H. pylori, restore the suppression of Th...Continue Reading

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Citations

Sep 13, 2020·Helicobacter·Karen Robinson, Philippe Lehours
Feb 23, 2021·Frontiers in Microbiology·Mariagrazia PiscioneGabriella Mincione
Mar 27, 2021·Probiotics and Antimicrobial Proteins·Shima Moradi-KalbolandiLeila Farahmand
Sep 28, 2021·Critical Reviews in Microbiology·Cláudia SousaLuís D R Melo

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