Oral immunization with an attenuated vaccine strain of Salmonella typhimurium expressing the serine-rich Entamoeba histolytica protein induces an antiamebic immune response and protects gerbils from amebic liver abscess.

Infection and Immunity
T Zhang, S L Stanley

Abstract

Attenuated salmonellae represent attractive candidates for the delivery of foreign antigens by oral vaccination. In this report, we describe the high-level expression of a recombinant fusion protein containing the serine-rich Entamoeba histolytica protein (SREHP), a protective antigen derived from virulent amebae, and a bacterially derived maltose-binding protein (MBP) in an attenuated strain of Salmonella typhimurium. Mice and gerbils immunized with S. typhimurium expressing SREHP-MBP produced mucosal immunoglobulin A antiamebic antibodies and serum immunoglobulin G antiamebic antibodies. Gerbils vaccinated with S typhimurium SREHP-MBP were protected against amebic liver abscess, the most common extraintestinal complication of amebiasis. Our findings indicate that the induction of mucosal and immune responses to the amebic SREHP antigen is dependent on the level of SREHP-MBP expression in S. typhimurium and establish that oral vaccination with SREHP can produce protective immunity to invasive amebiasis.

References

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Citations

Feb 6, 2007·Parasitology·S L Stanley
Nov 3, 1998·Parasite Immunology·L Ortiz-OrtizD M Rosales-Borjas
Dec 19, 2007·Infection and Immunity·Jose E Teixeira, Christopher D Huston
Oct 14, 2005·Expert Review of Vaccines·Omer A Chaudhry, William A Petri
Jun 20, 2007·Parasite Immunology·J C CarreroJ Morales-Montor
Feb 8, 2014·Human Vaccines & Immunotherapeutics·Jeanie QuachKris Chadee
Jan 28, 2021·Trends in Parasitology·Md Jashim UddinWilliam A Petri
Oct 23, 1997·Clinical Microbiology Reviews·S L Stanley
Sep 15, 2000·Infectious Disease Clinics of North America·M A Hughes, W A Petri
Sep 29, 2007·International Journal of Medical Microbiology : IJMM·Hannelore LotterEgbert Tannich

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