Oral immunization with cell-free self-assembly virus-like particles against orange-spotted grouper nervous necrosis virus in grouper larvae, Epinephelus coioides

Veterinary Immunology and Immunopathology
Ming-Hui ChienCheng-Hui Lin

Abstract

Nervous necrosis virus (NNV) infection causes viral nervous necrosis, inflicting serious economic losses in marine fish cultivation. Vaccination is the most effective choice for controlling and preventing viral infection. Virus-like particles (VLPs) are considered a novel vaccine platform because they are not infectious and they induce neutralizing antibodies efficiently. In the present study, we investigated the effect of the recombinant orange-spotted grouper NNV (OSGNNV) capsid proteins produced in Escherichia coli and cell-free self-assembled into VLPs on protective immune responses in orange-spotted grouper following immersion, intramuscular injection and oral immunization. We found the OSGNNV VLPs elicited neutralizing antibody with high efficacy, and provided the fish with full protection against OSGNNV challenge. In addition, the cell-free self-assembled OSGNNV VLPs did not contain residual host cell components and was safer compared with the intracellular assembled VLPs. Thus, oral vaccination is a more convenient and preferred route for fish vaccination. Our results show that the fish fed four times with a diet supplemented with 50-200 μg/g OSGNNV VLPs at 7-day intervals have sufficient protection. These findings demo...Continue Reading

Citations

Oct 18, 2019·Biomaterials Science·Xiankang HuJianxiang Zhang
Feb 27, 2020·International Journal of Molecular Sciences·Hsuan-Ju LinHong-Ting Victor Lin
Feb 14, 2020·Pathogens·Isabel Bandín, Sandra Souto
Jan 12, 2021·Frontiers in Bioengineering and Biotechnology·Mingming ZhangDawei Li
Apr 1, 2021·Developmental and Comparative Immunology·Irene SalinasJ Oriol Sunyer
Aug 31, 2021·Frontiers in Plant Science·Hang SuJihong Liu Clarke

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