Oral Microbiome and Gingival Tissue Apoptosis and Autophagy Transcriptomics.

Frontiers in Immunology
Jeffrey EbersoleOctavio A. Gonzalez

Abstract

Objective: This study focused on documenting characteristics of the gingival transcriptome during various stages of periodontitis targeting genes associated with apoptotic and autophagic pathways and changes that specifically associate with features of the oral microbiome. Methods: Macaca mulatta (n = 18; 12-23 years) were examined at baseline and 0.5, 1, and 3 months of disease progression, as well as 5 months with clinical disease resolution. 16S sequencing and microarray analyses examined changes in the microbiome and gingival transcriptome, respectively, at each time point from every animal. Results: Specific patterns of apoptotic and autophagic genes were identified related to the initiation and progression of disease. The analysis also provided insights on the principal bacteria within the complex microbiome whose abundance was significantly correlated with differences in apoptotic and autophagic gene expression. Bacteria were identified that formed associated complexes with similar effects on the host gene expression profiles. A complex of Leptotrichia_unclassifed, Capnocytophaga_unclassified, Prevotella sp. 317, and Veillonellaceae_[G-1] sp. 155 were significantly negatively correlated with both apoptosis and autophagy....Continue Reading

References

Jan 1, 1974·Acta Odontologica Scandinavica·K OhlssonG Tynelius-Bratthall
May 1, 1997·Journal of Clinical Periodontology·I L Chapple
Sep 2, 1998·Annals of Periodontology·R I GarciaP S Vokonas
Aug 18, 2005·Journal of Periodontal Research·C C TsaiC C Hung
Nov 8, 2005·Journal of Clinical Microbiology·Jørn A AasFloyd E Dewhirst
Nov 30, 2005·Immunological Reviews·Hiroshi TakayanagiTadatsugu Taniguchi
Aug 26, 2006·Periodontology 2000·Bruce J PasterFloyd E Dewhirst
Jan 12, 2007·Periodontology 2000·Iain L C Chapple, John B Matthews
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Paulo Henrique RodriguesAnn Progulske-Fox
Mar 19, 2008·Archives of Oral Biology·Esra BaltacioğluErdem Karabulut
May 2, 2008·Clinical and Vaccine Immunology : CVI·J L EbersoleM J Novak
Jul 4, 2008·Journal of Periodontology·Grishondra L Branch-MaysM John Novak
Oct 22, 2008·Nutrition·Mark A ReynoldsM John Novak
Jun 16, 2009·Cell·Jorge Moscat, Maria T Diaz-Meco
Feb 11, 2010·Cell·Noboru MizushimaBeth Levine
Mar 25, 2010·Molecular Oral Microbiology·A MekaL Kesavalu
Jan 21, 2011·Nature·Beth LevineHerbert W Virgin
Apr 8, 2011·Journal of Dental Research·O A GonzálezJ L Ebersole
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Aug 24, 2011·Journal of Periodontology·Poliana Mendes DuarteFernanda Vieira Ribeiro
Oct 7, 2011·Archives of Oral Biology·Poliana M DuarteAna Paula D Demasi
Jan 22, 2013·Apoptosis : an International Journal on Programmed Cell Death·Octavio A GonzalezJeffrey L Ebersole
May 3, 2013·Journal of Periodontology·Thomas E Van Dyke, Arie Jan van Winkelhoff
Dec 7, 2013·Journal of Clinical Periodontology·Octavio A GonzalezJeffrey L Ebersole
Jul 1, 2014·Journal of Clinical Periodontology·Jeffrey L EbersoleOctavio A Gonzalez
Nov 16, 2014·Applied and Environmental Microbiology·Mariana E KirstGary P Wang
Apr 6, 2016·Autophagy·Michaela Mrschtik, Kevin M Ryan
Mar 9, 2018·Journal of Dental Research·J Solbiati, J Frias-Lopez
Oct 12, 2018·Nature Reviews. Microbiology·Richard J LamontGeorge Hajishengallis
Mar 20, 2019·Infection and Immunity·Sreenatha KirakoduJeffrey Ebersole
Feb 23, 2020·Journal of Dental Research·J EbersoleO A Gonzalez
May 10, 2020·Periodontology 2000·Mike A CurtisThomas E Van Dyke

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Datasets Mentioned

BETA
PRJNA516659
E-MTAB-1977

Methods Mentioned

BETA
biopsies

Software Mentioned

ArrayExpress
Partek Genomics Suite

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