Oral Prion Neuroinvasion Occurs Independently of PrPC Expression in the Gut Epithelium

Journal of Virology
Alison MarshallN A Mabbott

Abstract

The early replication of certain prion strains within Peyer's patches in the small intestine is essential for the efficient spread of disease to the brain after oral exposure. Our data show that orally acquired prions utilize specialized gut epithelial cells known as M cells to enter Peyer's patches. M cells express the cellular isoform of the prion protein, PrPC, and this may be exploited by some pathogens as an uptake receptor to enter Peyer's patches. This suggested that PrPC might also mediate the uptake and transfer of prions across the gut epithelium into Peyer's patches in order to establish infection. Furthermore, the expression level of PrPC in the gut epithelium could influence the uptake of prions from the lumen of the small intestine. To test this hypothesis, transgenic mice were created in which deficiency in PrPC was specifically restricted to epithelial cells throughout the lining of the small intestine. Our data clearly show that efficient prion neuroinvasion after oral exposure occurred independently of PrPC expression in small intestinal epithelial cells. The specific absence of PrPC in the gut epithelium did not influence the early replication of prions in Peyer's patches or disease susceptibility. Acute muco...Continue Reading

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Citations

Feb 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kristina Endres
Oct 8, 2020·International Journal of Molecular Sciences·Neil A MabbottDavid S Donaldson

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Methods Mentioned

BETA
IEC
electrophoresis
PCR

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