Oral squamous carcinoma cells promote macrophage polarization in an MIF-dependent manner

QJM : Monthly Journal of the Association of Physicians
M Barbosa de Souza RizzoR A Mitchell

Abstract

Tumor-associated macrophages (TAMs) are important determinants of intratumoral immune evasion, neoangiogenesis, extracellular matrix remodeling and dysregulated tumor cell proliferation. Our prior studies revealed that macrophage-derived, but not tumor cell-derived, macrophage migration inhibitory factor (MIF), is an important determinant of TAM alternative activation and M2 polarization. Because MIF is historically thought to initiate signaling via a receptor-dependent, outside-in mode of action, we wished to investigate the specific contributions of tumor-derived vs. macrophage-derived MIF to M2 marker expression during macrophage polarization. Murine oral squamous cell-carcinoma cells (SCCVII) were co-cultured with either the RAW 264.7 mouse macrophage cell line or mouse primary bone marrow-derived macrophages in the context of MIF genetic loss/inhibition individually or in combination each cell type. Twelve well Transwell plates were used to co-culture SCCVII cells and RAW 264.7, MIF+/+ or MIF-/- macrophages treated with/without the small molecule MIF inhibitor, 4-iodo-6-phenylpyrimidine and incubated in the presence or absence of interleukin (IL-4) for 48 h. Macrophages were analyzed by quantitative real-time polymerase ch...Continue Reading

References

Jul 1, 1982·British Journal of Cancer·D G HirstJ L Hazlehurst
Jun 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E S WhiteD A Arenberg
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert A MitchellRichard Bucala
Dec 20, 2002·Nature·Lisa M Coussens, Zena Werb
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sergei Kusmartsev, Dmitry I Gabrilovich
Dec 19, 2008·Journal of Medicinal Chemistry·Zoe CourniaWilliam L Jorgensen
Dec 1, 2009·British Dental Journal·S Warnakulasuriya
Jan 27, 2012·Trends in Immunology·Brian RuffellLisa M Coussens
Nov 6, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kendra D SimpsonJanet V Cross
Feb 8, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kavitha YaddanapudiRobert A Mitchell
Jul 19, 2014·Immunity·Roy Noy, Jeffrey W Pollard
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
May 20, 2015·Nature Cell Biology·Bruno Costa-SilvaDavid Lyden
Nov 26, 2015·Cancer Immunology Research·Kavitha YaddanapudiRobert A Mitchell
Nov 5, 2016·Blood·Alejandra Gutiérrez-GonzálezPaloma Sánchez-Mateos
Apr 12, 2017·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Yuko YamagataKou Kayamori

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