Oral Typhoid Vaccination With Live-Attenuated Salmonella Typhi Strain Ty21a Generates Ty21a-Responsive and Heterologous Influenza Virus-Responsive CD4+ and CD8+ T Cells at the Human Intestinal Mucosa

The Journal of Infectious Diseases
Shaun H PenningtonMelita A Gordon

Abstract

Oral vaccination with live-attenuated Salmonella Typhi strain Ty21a is modestly efficacious, but the mechanisms of protection are currently unknown. While humoral and cellular immune responses are well described in peripheral blood, the cellular response at the intestinal mucosa has never been directly assessed. We vaccinated healthy adults with Ty21a and assessed humoral and cellular immunity in vaccinated volunteers and controls after 18 days. Immunoglobulin levels were assessed in peripheral blood by an enzyme-linked immunosorbent assay. Cellular responses were assessed in peripheral blood and at the duodenal and colonic mucosa by flow cytometry. We demonstrate the generation of Ty21a-responsive and heterologous influenza virus-responsive CD4(+) and CD8(+) T cells at the duodenal mucosa. All duodenal responses were consistently correlated, and no responses were observed at the colonic mucosa. Peripheral anti-lipopolysaccharide immunoglobulin G and immunoglobulin A responses were significantly correlated with duodenal responses. The assessment of integrin β7 expression intensity among peripheral and duodenal T-cell subsets revealed varied capacities for mucosal homing and residence. The breadth of duodenal cellular responses ...Continue Reading

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Citations

Aug 14, 2020·Proceedings of the National Academy of Sciences of the United States of America·Lorena Preciado-LlanesAlison Simmons
Jan 1, 2018·Allergo Journal : interdisziplinäre Zeitschrift für Allergologie und Umweltmedizin : Organ der Deutschen Gesellschaft für Allergie- und Immunitätsforschung·Emanuel PuschChrysanthi Skevaki
Oct 28, 2017·Frontiers in Immunology·Christoph J BlohmkeAndrew J Pollard
Jan 1, 2018·Allergo Journal International·Emanuel PuschChrysanthi Skevaki

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