Oral vaccination with the porcine rotavirus VP4 outer capsid protein expressed by Lactococcus lactis induces specific antibody production.

Journal of Biomedicine & Biotechnology
Yi-jing LiLi-wei Liu

Abstract

The objective of this study to design a delivery system resistant to the gastrointestinal environment for oral vaccine against porcine rotavirus. Lactococcus lactis NZ9000 was transformed with segments of vP4 of the porcine rotavirus inserted into the pNZ8112 surface-expression vector, and a recombinant L. lactis expressing VP4 protein was constructed. An approximately 27 kDa VP4 protein was confirmed by SDS-PAGE , Western blot and immunostaining analysis. BALB/c mice were immunized orally with VP4-expression recombinant L. lactis and cellular, mucosal and systemic humoral immune responses were examined. Specific anti-VP4 secretory IgA and IgG were found in feces, ophthalmic and vaginal washes and in serum. The induced antibodies demonstrated neutralizing effects on porcine rotavirus infection on MA104 cells. Our findings suggest that oral immunization with VP4-expressing L. lactis induced both specific local and systemic humoral and cellular immune responses in mice.

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Citations

Sep 9, 2011·Molecular Biotechnology·Shirin Tarahomjoo
Jan 17, 2013·Human Vaccines & Immunotherapeutics·Qinghua YuQian Yang
Dec 2, 2011·Annual Review of Food Science and Technology·Jerry Wells

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Methods Mentioned

BETA
ELISA
PCR
electrophoresis

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