Orally administered [¹⁴C]DPA and [¹⁴C]DHA are metabolised differently to [¹⁴C]EPA in rats

The British Journal of Nutrition
Gunveen KaurAndrew J Sinclair

Abstract

Previous studies have revealed that C20 PUFA are significantly less oxidised to CO₂ in whole-body studies compared with SFA, MUFA and C18 PUFA. The present study determined the extent to which three long-chain PUFA, namely 20:5n-3 EPA, 22:5n-3 docosapentaenoic acid (DPA) and 22:6n-3 DHA, were catabolised to CO₂ or, conversely, incorporated into tissue lipids. Rats were administered a single oral dose of 2·5 μCi [1-¹⁴C]DPA, [1-¹⁴C]EPA, [1-¹⁴C]DHA or [1-¹⁴C]oleic acid (18:1n-9; OA), and were placed in a metabolism chamber for 6 h where exhaled ¹⁴CO₂ was trapped and counted for radioactivity. Rats were euthanised after 24 h and tissues were removed for analysis of radioactivity in tissue lipids. The results showed that DPA and DHA were catabolised to CO₂ significantly less compared with EPA and OA (P<0·05). The phospholipid (PL) fraction was the most labelled for all three n-3 PUFA compared with OA in all tissues, and there was no difference between C20 and C22 n-3 PUFA in the proportion of label in the PL fraction. The DHA and DPA groups showed significantly more label than the EPA group in both skeletal muscle and heart. In the brain and heart tissue, there was significantly less label in the cholesterol fraction from the C22 n-...Continue Reading

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Citations

Nov 19, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Samaneh Ghasemi FardAndrew J Sinclair
Jun 23, 2015·Lipid Technology·Oleksandr A ByelashovGunveen Kaur
Feb 24, 2016·Nutrients·Michael J WeiserM Hasan Mohajeri
Dec 12, 2018·Journal of Lipid Research·Poorna C R YalagalaPapasani V Subbaiah
Jun 16, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Caroline MorinSamuel Fortin
Jun 30, 2019·Nutrients·Stefano CarboniDavid Lee Hamilton
Oct 26, 2021·Plant Biotechnology Journal·Srinivas BelideXue-Rong Zhou

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