ORC is necessary at the interphase-to-mitosis transition to recruit cdc2 kinase and disassemble RPA foci

Current Biology : CB
O CuvierMarcel Méchali

Abstract

The origin-recognition complex (ORC) has an essential role in defining DNA replication origins and in chromosome segregation. Recent studies in Drosophila orc2 mutants, and in human cells depleted of ORC2, have suggested that this factor is also implicated in mitotic chromosome assembly. We asked whether ORC was required for M phase chromosome assembly independently of its function in DNA replication. We performed depletion assays and reconstitution experiments in Xenopus egg extracts, in conditions of M phase chromosome assembly coupled or uncoupled from DNA replication. We show that, although ORC is dispensable for mitotic chromosome condensation, it is necessary at the interphase-mitosis transition for proper mitotic chromosome assembly to occur in a reaction not strictly dependent on DNA replication. This function involves the recruitment to chromatin of cdc2 kinase and the chromatin disassembly of interphasic replication protein A (RPA) foci. Furthermore, we show that mutations of RPA at the cdc2 kinase site prevents RPA dissociation from chromatin and impairs mitotic chromosome assembly without affecting DNA replication. Our results support the conclusion that in addition to its role in the assembly of prereplication comp...Continue Reading

References

Nov 30, 1990·Cell·M J SolomonM W Kirschner
May 25, 1989·Nature·A W Murray, M W Kirschner
Jun 1, 1995·Molecular and Cellular Biology·D M GilbertM L DePamphilis
Apr 1, 1997·Current Opinion in Genetics & Development·A Rowles, J J Blow
Feb 8, 2000·The Journal of Biological Chemistry·P RomanowskiR A Laskey
Jun 5, 2002·Annual Review of Biochemistry·Stephen P Bell, Anindya Dutta
Aug 10, 2002·Science·Supriya G PrasanthBruce Stillman
Jan 14, 2003·Nature Cell Biology·Mark JackmanJonathon Pines
Feb 27, 2003·The Journal of Cell Biology·Olivier Cuvier, Tatsuya Hirano
Mar 19, 2003·Biochemistry·Gregory G OakleyKathleen Dixon
Apr 2, 2003·Molecular Cell·Jason R Swedlow, Tatsuya Hirano
Apr 4, 2003·Current Opinion in Genetics & Development·Brian McHugh, Margarete M S Heck
May 10, 2003·Science·Jonathan D MooreTim Hunt
Feb 18, 2004·Molecular and Cellular Biology·Vitaly M VassinJames A Borowiec
Sep 3, 2004·Current Biology : CB·Peter J Gillespie, Tatsuya Hirano
Sep 28, 2004·Nature Cell Biology·Tatsuro S TakahashiJohannes C Walter
Oct 1, 2004·Journal of Cell Science·Patricia FrançonMarcel Méchali
Mar 9, 2005·The Journal of Cell Biology·Mark G Alexandrow, Joyce L Hamlin

❮ Previous
Next ❯

Citations

Aug 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Supriya G PrasanthBruce Stillman
May 13, 2008·The Journal of Biological Chemistry·Stuart J HaringMarc S Wold
Jun 3, 2008·The Journal of Biological Chemistry·Sara K Binz, Marc S Wold
Apr 3, 2008·Genes & Development·Olivier CuvierMarcel Mechali
Jan 5, 2011·Genes & Development·Atsuya NishiyamaMarcel Méchali
Apr 4, 2013·BMC Molecular Biology·Nicholas W AshtonDerek J Richard
May 1, 2007·Current Opinion in Cell Biology·Takayo Sasaki, David M Gilbert
Oct 11, 2012·Biochemical and Biophysical Research Communications·Bénédicte Recolin, Domenico Maiorano
May 15, 2013·Bioscience Reports·Zhen Shen
Apr 16, 2009·Critical Reviews in Biochemistry and Molecular Biology·Derek J RichardKum Kum Khanna
May 13, 2020·Briefings in Functional Genomics·Mohd AmirMd Imtaiyaz Hassan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.