Orcokinins regulate the expression of neuropeptide precursor genes related to ecdysis in the hemimetabolous insect Rhodnius prolixus

Journal of Insect Physiology
Juan Pedro WulffSheila Ons

Abstract

Ecdysis is a vital process for insects, during which they shed the old cuticle in order to emerge as the following developmental stage. Given its relevance for survival and reproduction, ecdysis is tightly regulated by peptidic hormones that conform an interrelated neuromodulatory network. This network was studied in species that undergo a complete metamorphosis, but not in hemimetabola. In a recent work, we demonstrated that orcokinin neuropeptides are essential for ecdysis to occur in the kissing bug Rhodnius prolixus. Here we performed gene silencing, quantitative PCR and in vitro treatments in order to study the interrelationships between RhoprOKs and hormones such as ecdysis triggering hormone, corazonin, eclosion hormone, crustacean cardioactive peptide and ecdysone. Our results suggest that RhoprOKs directly or indirectly regulate the expression of other genes. Whereas RhoprOKA is centrally involved in the regulation of gene expression, RhoprOKB is implicated in processes related to midgut physiology. Therefore, we propose that the different transcripts encoded in RhoprOK gene could integrate signaling cues, in order to coordinate the nutritional state with development and ecdysis. Given the emerging data that point to O...Continue Reading

Citations

May 6, 2019·The Journal of Experimental Biology·Natalia CapriottiSheila Ons
Mar 21, 2020·Frontiers in Physiology·Kai WuErjun Ling
Nov 24, 2018·Scientific Reports·Andrés LavoreSheila Ons
Oct 31, 2020·Current Biology : CB·Elisabeth ZiegerAndreas Wanninger
Jul 17, 2021·Evolution; International Journal of Organic Evolution·Elisabeth ZiegerAndreas Wanninger

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