PMID: 6111337Feb 3, 1981Paper

Ordered conformation of polypeptides and proteins in acidic dodecyl sulfate solution

Biochemistry
C S WuJ T Yang

Abstract

The conformation of some polypeptides and proteins in sodium dodecyl sulfate (NaDodSO4) solutions was studied by circular dichroism. The type and extent of induced structure depend on their helix- and beta-forming potential. Anionic side groups in segments of helix or beta form tend to destabilize the ordered structure unless they are protonated. beta-Endorphin has one Glu inside a predicted helical segment; its helicity in a NaDodSO4 solution is enhanced at pH below 4. alpha-Melanocyte-stimulating hormone having a Glu in a beta segment undergoes a pH-induced coil to beta transition in 1.25 mM NaDodSO4 (excess surfactant will disrupt the beta form). Reduced somatostatin assumes a beta form in 2 mM NaDodSO4 and a partial helix in 25 mM NaDodSO4, both of which are unchanged in acidic pH because it lacks -COOH groups. The unordered gastrin with five consecutive Glu's becomes helical in a NaDodSO4 solution at pH 4. Neurotensin with one Glu has no structure-forming potential and is unordered in both neutral and acidic NaDodSO4 solutions. This charge effect also manifests in segments of ordered structure for polypeptides and proteins such as glucagon, cytochrome c, parvalbumin, ribonuclease A, and lysozyme. The effect is especially p...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
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Oct 20, 1975·Biochimica Et Biophysica Acta·B W Matthews
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Citations

Aug 1, 1995·Biopolymers·D J ButcherG R Moe
Aug 1, 1991·Journal of Protein Chemistry·C S WuJ T Yang
Jul 1, 1990·Biochemical Pharmacology·R W Behling, L W Jelinski
Nov 14, 1998·Biochimica Et Biophysica Acta·Q X WuB A Baldo
Aug 10, 1999·Journal of the American Society for Mass Spectrometry·I D Figueroa, D H Russell
Feb 13, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·C SubbalakshmiN Sitaram
Sep 29, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·K MiyamotoY Kuroda
Feb 3, 2009·Nature Cell Biology·Joseph P YuanShmuel Muallem
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C M Deber, B A Behnam
May 25, 1992·Proceedings of the National Academy of Sciences of the United States of America·L Zhong, W C Johnson
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·K Dahlman-WrightT Härd
May 4, 2000·Protein Science : a Publication of the Protein Society·P V DubovskiiK Akasaka
Apr 9, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·Y W Yang, C C Teng
May 15, 1997·European Journal of Biochemistry·S Albouz-AboM von Itzstein
Jul 14, 2001·Antimicrobial Agents and Chemotherapy·J Bestman-SmithM G Bergeron
Aug 18, 2004·Molecular and Cellular Biology·Kleber P MadridArmando Jardim
Oct 27, 2004·Bioscience, Biotechnology, and Biochemistry·Joel CornistaShigenori Kanaya

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