Ordered recruitment of chromatin modifying and general transcription factors to the IFN-beta promoter
Here, we show that the IFN-beta enhanceosome activates transcription by directing the ordered recruitment of chromatin modifying and general transcription factors to the IFN-beta promoter. The enhanceosome is assembled in the nucleosome-free enhancer region of the IFN-beta gene, leading to the modification and remodeling of a strategically positioned nucleosome that masks the TATA box and the start site of transcription. Initially, the GCN5 complex is recruited, which acetylates the nucleosome, and this is followed by recruitment of the CBP-PolII holoenzyme complex. Nucleosome acetylation in turn facilitates SWI/SNF recruitment by CBP, resulting in chromatin remodeling. This program of recruitment culminates in the binding of TFIID to the promoter and the activation of transcription.
Location analysis of DNA-bound proteins at the whole-genome level: untangling transcriptional regulatory networks
IRF-1 and NF-kappaB p50/cRel bind to distinct regions of the proximal murine IL-12 p35 promoter during costimulation with IFN-gamma and LPS
Functional interplay between CBP and PCAF in acetylation and regulation of transcription factor KLF13 activity
Promoter-specific regulation of MyoD binding and signal transduction cooperate to pattern gene expression
Dynamic chromatin states in human ES cells reveal potential regulatory sequences and genes involved in pluripotency
TNF activates an IRF1-dependent autocrine loop leading to sustained expression of chemokines and STAT1-dependent type I interferon-response genes
Differential innate immune response programs in neuronal subtypes determine susceptibility to infection in the brain by positive-stranded RNA viruses
Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter
Detection of factors that interact with the human beta-interferon regulatory region in vivo by DNAase I footprinting
Nucleosome positioning on the MMTV LTR results from the frequency-biased occupancy of multiple frames
Reversal of intrinsic DNA bends in the IFN beta gene enhancer by transcription factors and the architectural protein HMG I(Y)
Intermediates in formation and activity of the RNA polymerase II preinitiation complex: holoenzyme recruitment and a postrecruitment role for the TATA box and TFIIB
Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter
The IFN-beta enhancer: a paradigm for understanding activation and repression of inducible gene expression
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CZI Human Cell Atlas Seed Network
The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.
CREs: Gene & Cell Therapy
Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.