Ordered water molecules as key allosteric mediators in a cooperative dimeric hemoglobin

Proceedings of the National Academy of Sciences of the United States of America
William E RoyerJ M Friedman

Abstract

One of the most remarkable structural aspects of Scapharca dimeric hemoglobin is the disruption of a very well-ordered water cluster at the subunit interface upon ligand binding. We have explored the role of these crystallographically observed water molecules by site-directed mutagenesis and osmotic stress techniques. The isosteric mutation of Thr-72-->Val in the interface increases oxygen affinity more than 40-fold with a surprising enhancement of cooperativity. The only significant structural effect of this mutation is to destabilize two ordered water molecules in the deoxy interface. Wild-type Scapharca hemoglobin is strongly sensitive to osmotic conditions. Upon addition of glycerol, striking changes in Raman spectrum of the deoxy form are observed that indicate a transition toward the liganded form. Increased osmotic pressure, which lowers the oxygen affinity in human hemoglobin, raises the oxygen affinity of Scapharca hemoglobin regardless of whether the solute is glycerol, glucose, or sucrose. Analysis of these results provides an estimate of six water molecules lost upon oxygen binding to the dimer, in good agreement with eight predicted from crystal structures. These experiments suggest that the observed cluster of int...Continue Reading

References

Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J Badger, D L Caspar
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·Q H Gibson
Jan 14, 1994·Journal of Molecular Biology·W E Royer
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·T N BhatR J Poljak
Jan 1, 1995·Methods in Enzymology·C R Robinson, S G Sligar
Jan 1, 1995·Methods in Enzymology·V A ParsegianD C Rau
Mar 1, 1996·The Journal of Biological Chemistry·M F Colombo, G O Bonilla-Rodriguez
Oct 1, 1958·Archives of Biochemistry and Biophysics·A ROSSI-FANELLI, E ANTONINI
Jan 1, 1964·Advances in Protein Chemistry·J WYMAN
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX

❮ Previous
Next ❯

Citations

Jun 10, 2011·Journal of Biosciences·R SabarinathanK Sekar
Jan 1, 1997·Progress in Biophysics and Molecular Biology·M BolognesiP Ascenzi
Nov 11, 1998·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·V Norris
May 10, 2001·Trends in Biochemical Sciences·W E RoyerH A Heaslet
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·D Kültz, D Chakravarty
Nov 14, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·N HellmannH Decker
Aug 28, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Christian HundahlRoy E Weber
Dec 12, 2012·Quarterly Reviews of Biophysics·Pengyu RenNathan A Baker
Dec 21, 2006·Biochemistry·Jeffry C NicholsQuentin H Gibson
Nov 16, 2007·Biochemistry·Karin NienhausG Ulrich Nienhaus
Apr 21, 2009·Journal of the American Chemical Society·Hongtao Yu, Steven W Rick
Jul 17, 2009·Journal of the American Chemical Society·Feng Guo, Joel M Friedman
Nov 10, 1998·Nature Structural Biology·N K SauterD A Agard
Sep 7, 2006·Nature Reviews. Molecular Cell Biology·Martin Chaplin
Feb 22, 2013·Physical Chemistry Chemical Physics : PCCP·Daniela DrescherJanina Kneipp
May 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Thomas E AngelKrzysztof Palczewski
Dec 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Zhong RenWilliam E Royer
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sayan GuptaMark R Chance
Jan 22, 2008·The Journal of Biological Chemistry·José A GaviraJuan Manuel García-Ruiz
Dec 14, 2012·Journal of the Royal Society, Interface·Panagiotis L Kastritis, Alexandre M J J Bonvin
Dec 2, 2006·Acta Crystallographica. Section D, Biological Crystallography·Ursula D Ramirez, Douglas M Freymann
Oct 30, 2004·European Journal of Biochemistry·Patricia PeresGustavo O Bonilla-Rodriguez
Aug 17, 2011·The Journal of Chemical Physics·Ramachandran GnanasekaranDavid M Leitner
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·R A WattsE S Dennis
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·V A ParsegianD C Rau
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·E L CunninghamD A Agard
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M CoutureM Guertin
May 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·James E KnappWilliam E Royer
Nov 3, 2010·Biochimica Et Biophysica Acta·Alessio Peracchi, Andrea Mozzarelli
Apr 15, 2008·Advances in Colloid and Interface Science·Vuk Uskoković
Nov 24, 2004·Respiratory Physiology & Neurobiology·Roy E Weber, Angela Fago
Dec 22, 2006·Biochimica Et Biophysica Acta·Stefan FranzenJennifer L Belyea
Jan 18, 2008·Biophysical Journal·Christopher B Stanley, Helmut H Strey
Mar 26, 2013·Biochimica Et Biophysica Acta·Luca RondaStefano Bettati
Apr 12, 2016·The Journal of Physical Chemistry. B·David M Leitner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.