Orexigenic effect of cocaine- and amphetamine-regulated transcript (CART) after injection into hypothalamic nuclei in streptozotocin-diabetic rats

Clinical and Experimental Pharmacology & Physiology
Jun HouShi-Wen Zhou

Abstract

1. The aim of the present study was to investigate the orexigenic effect of cocaine- and amphetamine-regulated transcript (CART) peptide on feeding regulation following its injection into discrete nuclei of the hypothalamus. 2. Male Sprague-Dawley diabetic rats were injected with 0.06 or 0.2 nmol CART (55-102) or an equal volume of saline into various hypothalamic areas and food intake was then measured 1, 2, 4, 8 and 24 h after injection. Changes in hypothalamic CART mRNA expression in response to dietary intervention (2 weeks feeding of a high-fat diet) were assessed using quantitative real-time reverse transcription-polymerase chain reaction. Possible interactions between neuropeptide Y (NPY), agouti-related protein (AGRP), α-melanocyte-stimulating hormone (MSH) and corticotropin-releasing hormone (CRH) were evaluated in an in vitro hypothalamic explant system. Neuropeptide immunoreactivities (IR) were determined using radioimmunoassays (RIAs). 3. At 0.2 nmol, CART (55-102) significantly increased feeding in fasted diabetic rats after injection into the dorsomedial hypothalamic nucleus and arcuate nucleus (ARC). Injection of 0.2 nmol CART (55-102) into the ARC of satiated diabetic rats also increased food intake that was sim...Continue Reading

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Citations

Jan 29, 2014·Peptides·Nishikant K SubhedarDadasaheb M Kokare
Aug 30, 2013·Canadian Journal of Physiology and Pharmacology·Jun HouYonghe Hu
Sep 21, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shin J LeeKevin L Grove
Oct 30, 2014·Frontiers in Neuroscience·Jackie Lau, Herbert Herzog
Feb 22, 2017·Endocrine Regulations·M M I Abdalla
Sep 1, 2017·Scientific Reports·Jackie LauHerbert Herzog
Nov 19, 2020·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christopher J HaddockGina L C Yosten
Apr 27, 2020·Brain Research·Zhi Yi Ong, Gavan P McNally
Mar 12, 2021·Physiology & Behavior·Gina L C YostenWillis K Samson

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