Orf Virus -Based Vaccine Vector D1701-V Induces Strong CD8+ T Cell Response against the Transgene but Not against ORFV-Derived Epitopes

Vaccines
Alena ReguzovaRalf Amann

Abstract

The potency of viral vector-based vaccines depends on their ability to induce strong transgene-specific immune response without triggering anti-vector immunity. Previously, Orf virus (ORFV, Parapoxvirus) strain D1701-V was reported as a novel vector mediating protection against viral infections. The short-lived ORFV-specific immune response and the absence of virus neutralizing antibodies enables repeated immunizations and enhancement of humoral immune responses against the inserted antigens. However, only limited information exists about the D1701-V induced cellular immunity. In this study we employed major histocompatibility complex (MHC) ligandomics and immunogenicity analysis to identify ORFV-specific epitopes. Using liquid chromatography-tandem mass spectrometry we detected 36 ORFV-derived MHC I peptides, originating from various proteins. Stimulated splenocytes from ORFV-immunized mice did not exhibit specific CD8+ T cell responses against the tested peptides. In contrast, immunization with ovalbumin-expressing ORFV recombinant elicited strong SIINFEKL-specific CD8+ T lymphocyte response. In conclusion, our data indicate that cellular immunity to the ORFV vector is negligible, while strong CD8+ T cell response is induced ...Continue Reading

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Citations

Nov 12, 2020·The Biochemical Journal·Chathura D SuraweeraMarc Kvansakul
Dec 3, 2020·Pathogens·Justyna Struzik, Lidia Szulc-Dąbrowska

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Methods Mentioned

BETA
transfection
PCR
flow cytometry
transgenic

Software Mentioned

Proteome Discoverer
NetMHCpan4
ImmunoSpot
GraphPad
Sequest HT
GraphPad Prism
FlowJo

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