ORF4 of the Temperate Archaeal Virus SNJ1 Governs the Lysis-Lysogeny Switch and Superinfection Immunity.

Journal of Virology
Beibei ChenShishen Du

Abstract

Recent environmental and metagenomic studies have considerably increased the repertoire of archaeal viruses and suggested that they play important roles in nutrient cycling in the biosphere. However, very little is known about how they regulate their life cycles and interact with their hosts. Here, we report that the life cycle of the temperate haloarchaeal virus SNJ1 is controlled by the product ORF4, a small protein belonging to the antitoxin MazE superfamily. We show that ORF4 controls the lysis-lysogeny switch of SNJ1 and mediates superinfection immunity by repression of genomic DNA replication of the superinfecting viruses. Bioinformatic analysis shows that ORF4 is highly conserved in two SNJ1-like proviruses, suggesting that the mechanisms for lysis-lysogeny switch and superinfection immunity are conserved in this group of viruses. As the lysis-lysogeny switch and superinfection immunity of archaeal viruses have been poorly studied, we suggest that SNJ1 could serve as a model system to study these processes.IMPORTANCE Archaeal viruses are important parts of the virosphere. Understanding how they regulate their life cycles and interact with host cells provide crucial insights into their biological functions and the evoluti...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C SchleperW Zillig
Jan 1, 1989·Canadian Journal of Microbiology·S W ClineW F Doolittle
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R L CharleboisW F Doolittle
Apr 1, 1994·Trends in Microbiology·M J Lu, U Henning
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·E AizenmanG Glaser
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 30, 2003·Molecular Cell·Katsuhiko KamadaStephen K Burley
Sep 13, 2005·Journal of Molecular Biology·Caroline RanquetJohannes Geiselmann
Nov 16, 2005·Annual Review of Genetics·Amos B OppenheimSankar Adhya
Dec 22, 2006·PloS One·Sebastian BreuertJörg Soppa
Nov 21, 2007·Canadian Journal of Microbiology·Yunjun MeiXiangdong Chen
Nov 18, 2008·Journal of Molecular Biology·Vitold E GalkinEdward H Egelman
Nov 28, 2008·Journal of Virology·Timothy R Wagenaar, Bernard Moss
Dec 17, 2008·Methods in Molecular Biology·Sherwood R Casjens, Eddie B Gilcrease
Jan 18, 2013·BMC Bioinformatics·Jacques Oberto
Oct 4, 2013·Journal of Virology·Emmanuelle R J QueminMart Krupovic
Mar 13, 2014·FEMS Microbiology Reviews·Britt Koskella, Michael A Brockhurst
Mar 8, 2016·Trends in Microbiology·Sylvain Gandon
Jun 4, 2016·The ISME Journal·Joseph Bondy-DenomyKaren L Maxwell
Nov 25, 2016·Nature Communications·Carol SheppardFinn Werner
Jan 19, 2017·Nature·Zohar ErezRotem Sorek
Jun 18, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Arshan NasirGustavo Caetano-Anollés
Oct 7, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ellie Harrison, Michael A Brockhurst
Nov 11, 2017·Nature Reviews. Microbiology·David PrangishviliMart Krupovic
Nov 28, 2017·Virus Research·Mart KrupovicEugene V Koonin
Dec 21, 2017·Journal of Molecular Biology·Lukas ZimmermannVikram Alva
Jan 24, 2018·Nucleic Acids Research·Jiao WangXiangdong Chen
Apr 13, 2019·Nature Communications·Lei XiongShi Chen

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