OrfX, a Nucleomodulin Required for Listeria monocytogenes Virulence.

MBio
Andrzej ProkopOlivier Dussurget

Abstract

Listeria monocytogenes is a bacterial pathogen causing severe foodborne infections in humans and animals. Listeria can enter into host cells and survive and multiply therein, due to an arsenal of virulence determinants encoded in different loci on the chromosome. Several key Listeria virulence genes are clustered in Listeria pathogenicity island 1. This important locus also contains orfX (lmo0206), a gene of unknown function. Here, we found that OrfX is a small, secreted protein whose expression is positively regulated by PrfA, the major transcriptional activator of Listeria virulence genes. We provide evidence that OrfX is a virulence factor that dampens the oxidative response of infected macrophages, which contributes to intracellular survival of bacteria. OrfX is targeted to the nucleus and interacts with the regulatory protein RybP. We show that in macrophages, the expression of OrfX decreases the level of RybP, which controls cellular infection. Collectively, these data reveal that Listeria targets RybP and evades macrophage oxidative stress for efficient infection. Altogether, OrfX is after LntA, the second virulence factor acting directly in the nucleus.IMPORTANCEListeria monocytogenes is a model bacterium that has been ...Continue Reading

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Citations

Apr 28, 2018·International Journal of Environmental Research and Public Health·Agnieszka Chlebicz, Katarzyna Śliżewska
Mar 19, 2020·Cellular Microbiology·Francis Impens, Olivier Dussurget
Mar 19, 2020·Cellular Microbiology·Hélène Bierne, Mélanie Hamon
Apr 5, 2020·Toxins·Hélène Bierne, Renaud Pourpre
Jun 3, 2020·Proceedings of the National Academy of Sciences of the United States of America·Melanie BuretteMatteo Bonazzi
May 30, 2020·International Journal of Molecular Sciences·Lea DenzerChristian Schwerk
Jan 22, 2021·PLoS Pathogens·Hannah E HanfordYousef Abu Kwaik
Feb 16, 2021·Trends in Microbiology·Olivier DissonMarc Lecuit
Nov 3, 2020·Translational Oncology·Abdul Arif Khan, Zakir Khan
Dec 14, 2018·Microbiology Spectrum·Javier Pizarro-Cerdá, Pascale Cossart

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Methods Mentioned

BETA
fluorescence microscopy
reverse transcription-PCR
two-hybrid
confocal microscopy
Immunoprecipitation
immunoprecipitations
coimmunoprecipitation
transfection
PCR

Software Mentioned

Metamorph

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