Abstract
Rosmarinic acid (RA), a compound found in several plant species, has beneficial properties, including anti-inflammatory and antibacterial effects. We investigated the toxicity, anti-inflammatory, and antifibrotic effects of RA using precision-cut liver slices (PCLS) and precision-cut intestinal slices (PCIS) prepared from human, mouse, and rat tissue. PCLS and PCIS were cultured up to 48 h in the absence or presence of RA. Gene expression of the inflammatory markers: IL-6, IL-8/CXCL1/KC, and IL-1β, as well as the fibrosis markers: pro-collagen 1a1, heat shock protein 47, α-smooth muscle actin, fibronectin (Fn2) and plasminogen activator inhibitor-1 (PAI-1) were evaluated by qPCR. RA was only toxic in murine PCIS. RA failed to mitigate the inflammatory response in most models, while it clearly reduced IL-6 and CXCL1/KC gene expression in murine PCIS at non-toxic concentrations. With regard to fibrosis, RA decreased the gene levels of Fn2 and PAI-1 in murine PCLS, and Fn2 in murine PCIS. Yet, no effect was observed on the gene expression of fibrosis markers in human and rat PCIS. In conclusion, we observed clear organ- and species-specific effects of RA. RA had little influence on inflammation. However, our study further establis...Continue Reading
References
Dec 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·O K Mirzoeva, P C Calder
Dec 1, 1996·Journal of Ethnopharmacology·W KrolM Mos
Aug 29, 1998·Journal of Natural Products·T Nakazawa, K Ohsawa
Dec 17, 2002·Phytochemistry·Maike Petersen, Monique S J Simmonds
Apr 10, 2003·Free Radical Biology & Medicine·Chiaki SanbongiToshikazu Yoshikawa
May 4, 2004·Life Sciences·Seigo BabaJunji Terao
Aug 17, 2004·European Journal of Nutrition·Seigo BabaJunji Terao
Dec 19, 2006·Gut·F RiederG Rogler
May 15, 2007·Lancet·Daniel C Baumgart, Simon R Carding
May 13, 2008·Gastroenterology·Scott L Friedman
Jul 23, 2008·Toxicology·Hyo Jung LeeHong Sung Chun
Oct 22, 2008·The Journal of Nutrition·Kristen A ScheckelDonato F Romagnolo
Jun 16, 2009·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Gui-Sheng LiFeng-Hua Fu
Oct 20, 2009·Nutrition and Cancer·Cristina P R XavierCristina Pereira-Wilson
Apr 24, 2010·BMC Gastroenterology·Thierry PoynardJean Pierre Giordanella
Jul 29, 2010·Seminars in Liver Disease·Thomas A Wynn, Luke Barron
Aug 21, 2010·Nature Protocols·Inge A M de GraafGeny M M Groothuis
Nov 30, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·An-Chin ChengMin-Hsiung Pan
Jun 1, 2012·Molecular Nutrition & Food Research·Ankur KarmokarKaren Brown
Nov 3, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Robert DomitrovićMaja Bival Stefan
Dec 11, 2013·Toxicology and Applied Pharmacology·Inge M WestraPeter Olinga
Oct 8, 2014·Basic & Clinical Pharmacology & Toxicology·Joao RochaBruno Sepodes
Jan 30, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Natalie J TorokScott L Friedman
Apr 25, 2015·Physiological Reports·Bao Tung PhamPeter Olinga
Sep 18, 2015·Frontiers in Medicine·Henricus A M MutsaersPeter Olinga
Citations
May 29, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Inge M WestraPeter Olinga
Jul 5, 2016·European Journal of Pharmacology·Elisabeth G D StribosHenricus A M Mutsaers
Sep 13, 2017·The AAPS Journal·Mitchel J R RuigrokWouter L J Hinrichs
Nov 23, 2019·Archives of Toxicology·Emilia BigaevaPeter Olinga
Jul 12, 2017·British Journal of Pharmacology·Theerut LuangmonkongPeter Olinga
Mar 3, 2017·Scientific Reports·Huie LiXiaozhong Lan
Aug 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xuan CaiYonghong Lu
Aug 31, 2019·Oxidative Medicine and Cellular Longevity·Igor KumburovicGvozden Rosic
Jul 24, 2020·Pakistan Journal of Biological Sciences : PJBS·Hadeer Yahia DarwishAlaa Alnefaie
Jan 17, 2020·Inflammatory Bowel Diseases·Raditya IswandanaPeter Olinga
Aug 24, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Carin BielPeter Olinga
Jul 28, 2020·Food Chemistry·Siyu ZhangCasimir C Akoh
Mar 20, 2020·Current Pharmaceutical Biotechnology·Sarah AlbogamiAfnan Alattas
Aug 15, 2021·Plant Physiology and Biochemistry : PPB·Imane ChamkhiAbdelhakim Bouyahya
Feb 20, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Taiwo O Elufioye, Solomon Habtemariam