PMID: 1203246Dec 17, 1975Paper

Organ and intracellular localization of short-chain acyl-CoA synthetases in rat and guinea-pig

Biochimica Et Biophysica Acta
H R Scholte, P H Groot

Abstract

1. Homogenates of rat epididymal fat pad, heart, kidney, lactating mammary gland, liver, skeletal muscle and small intestinal mucosa have been partitioned into a particulate and supernatant fraction. With reliable marker enzymes for the mitochondrial matrix and the cytosol: propionyl-CoA carboxylase and pyruvate kinase, the distributions of the acyl-CoA synthetase activities measured at 1 and 10 mM C2, C3 and C4 over mitochondria and cytosol have been calculated. From these values an estimate was made of the K0.5 of the fatty acids. 2. A distinct fatty acid-activating enzyme was assumed to be present in one of the compartments when that fatty acid was activated with a K0.5 less than or equal to 1.5 mM in an amount of greater than 13% of the total cellular activity. Adipose tissue, gut, liver and mammary gland, all organs of a high lipogenetic capacity, contained a cytosolic acetyl-CoA synthetase. At 1 mM acetate 60, 31, 77 and 83% of the total cellular activities in these organs were cytosolic in nature, with activities of 0.021, 0.32, 0.37 and 1.16 mumol C2 activated per min per g wet weight, respectively. 3. Mitochondrial acetyl-CoA and butyryl-CoA synthetases were found in adipose tissue, gut, heart, kidney, mammary gland an...Continue Reading

References

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Citations

Feb 1, 1991·Cardiovascular Drugs and Therapy·W C Hülsmann
Feb 1, 1986·Journal of Chemical Ecology·D Morse, E Meighen
Mar 1, 1994·Chemico-biological Interactions·E P Brass
Jan 18, 1995·Biochimica Et Biophysica Acta·Q N DiepS Skrede
Nov 1, 1976·Acta Physiologica Scandinavica·N KarlssonK H Kiessling
Jan 19, 2011·Chembiochem : a European Journal of Chemical Biology·Brittany N AlbaughJohn M Denu
Apr 16, 2016·Journal of Lipid Research·Peter Schönfeld, Lech Wojtczak
Feb 15, 1992·European Journal of Biochemistry·C T KnudsenB Quistorff
Jul 1, 1988·Basic Research in Cardiology·K T KiviluomaI E Hassinen
Jan 19, 2020·Mechanisms of Ageing and Development·Pratishtha ChatterjeeRalph N Martins
Feb 28, 1986·Biochimica Et Biophysica Acta·C DemignéP Fafournoux
Mar 13, 1992·Biochimica Et Biophysica Acta·M J RaatikainenI E Hassinen

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