Organ-level analysis of idioblast patterning in Egeria densa Planch. leaves

PloS One
Takuya HaraMakoto T Fujiwara

Abstract

Leaf tissues of plants usually contain several types of idioblasts, defined as specialized cells whose shape and contents differ from the surrounding homogeneous cells. The spatial patterning of idioblasts, particularly of trichomes and guard cells, across the leaf epidermis has received considerable attention as it offers a useful biological model for studying the intercellular regulation of cell fate and patterning. Excretory idioblasts in the leaves of the aquatic monocotyledonous plant Egeria densa produced light blue autofluorescence when irradiated with ultraviolet light. The use of epifluorescence microscopy to detect this autofluorescence provided a simple and convenient method for detecting excretory idioblasts and allowed tracking of those cells across the leaf surfaces, enabling quantitative measurement of the clustering and spacing patterns of idioblasts at the whole leaf level. Occurrence of idioblasts was coordinated along the proximal-distal, medial-lateral, and adaxial-abaxial axes, producing a recognizable consensus spatial pattern of idioblast formation among fully expanded leaves. Idioblast clusters, which comprised up to nine cells aligned along the proximal-distal axis, showed no positional bias or regulari...Continue Reading

References

Jul 1, 1997·The Plant Cell·J C LarkinF Sack
Nov 25, 2005·Plant & Cell Physiology·Haruko UedaIkuko Hara-Nishimura
Dec 5, 2006·Current Opinion in Plant Biology·Cathie Martin, Beverley J Glover
Feb 1, 2007·Plant, Cell & Environment·Fei YuSteven Rodermel
Sep 13, 2013·Annals of Botany·Jean-Marc BrillouetGeneviève Conéjéro
Mar 1, 1988·The New Phytologist·Abraham Fahn

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Citations

Jun 14, 2018·Plant & Cell Physiology·Makoto Shirakawa, Ikuko Hara-Nishimura
Dec 17, 2020·International Journal of Molecular Sciences·Ekaterina N BaranovaAlexander A Gulevich
Feb 6, 2018·Trends in Plant Science·Maite Colinas, Alain Goossens

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Methods Mentioned

BETA
light microscopy
Fluorescence
fluorescence microscopy

Software Mentioned

Photoshop
Adobe Illustrator
Adobe Photoshop
ImageJ

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