Organ-specific gene expressions in C57BL/6 mice after exposure to low-dose radiation

Radiation Research
Woo-Jung LeeYun-Sil Lee

Abstract

The possibility that radiation-induced alterations in gene expression are tissue specific and are related to apoptosis was examined using samples from brain, heart, lung, spleen and intestine from female C57BL6 mice after exposure to 0.2 Gy radiation. Apoptosis was the highest in spleen and intestine, moderate in lung, and absent in brain and heart. However, the mRNA expression of Trp53 and Cdkn1a (p21) after irradiation was not different among the organ types, and immunohistochemistry revealed that all the organs expressed these two proteins after irradiation. When expression patterns of 23 genes in the organs were examined by RT-PCR, neogenine, Apo1, nuclease sensitive element binding protein 1, syntaxin, cyclin G1, hNOP56, paraoxonase and glutathione peroxidase were overexpressed after irradiation in all the organs sampled, suggesting them as universal exposure markers for low-dose radiation. Sialyltransferase may be a candidate for radiation detection in spleen and intestine, which are radiosensitive organs. Because Sod1 (Cu/ZnSOD) and alphaB crystalline were expressed only in spleen, and protein tyrosine kinase and platelet membrane glycoprotein lib were expressed in both spleen and lung, these genes may also be potential ...Continue Reading

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Citations

Apr 3, 2008·Journal of Biomedicine & Biotechnology·M Ahmad Chaudhry
Jun 21, 2014·Biochimica Et Biophysica Acta·Fabio Dall'OlioMariella Chiricolo
Feb 12, 2009·International Journal of Radiation Biology·G VaresI Hayata
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Mar 27, 2010·International Journal of Radiation Oncology, Biology, Physics·Minyoung LeeYun-Sil Lee
Oct 31, 2007·International Journal of Radiation Oncology, Biology, Physics·Hae-June LeeYun-Sil Lee
Jul 22, 2006·Biochemical and Biophysical Research Communications·Boris GavrilovNikolai Tomilin
May 30, 2019·International Journal of Radiation Biology·Stephanie PuukilaDani-Louise Dixon
Feb 26, 2016·Journal of Radiological Protection : Official Journal of the Society for Radiological Protection·Julie J BurttRobert M Lafrenie
Aug 24, 2010·Radiation Research·Molykutty John-AryankalayilC Norman Coleman
Mar 7, 2007·Journal of Radiation Research·Chander Mohan PathakSuresh Chander Sharma
Aug 19, 2008·Molecular Cancer Research : MCR·Minyoung LeeYun-Sil Lee
Dec 22, 2020·International Journal of Radiation Biology·Yeni Natalia C Perez-GelvezCarl W Bergmann
Nov 3, 2020·Frontiers in Genetics·Eunguk ShinBuHyun Youn

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Methods Mentioned

BETA
PCR
electrophoresis

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis