Organ variation in the mutagenicity of dimethylnitrosamine in Big Blue mice

Environmental and Molecular Mutagenesis
T SuzukiT Sofuni

Abstract

Organ specificity in the lacI mutant frequency (MF) induced by dimethylnitrosamine (DMN) was analyzed in lung, liver, kidney, bone marrow, urinary bladder, and testis of Big Blue mice. Cell proliferative activity was also analyzed in some of these tissues by immunohistochemical staining of proliferating cell nuclear antigen (PCNA). Clastogenicity of DMN was concomitantly analyzed by the peripheral blood micronucleus assay with the same animals used for the lacI mutation assay. Five daily intraperitoneal (i.p.) treatments with DMN (1 mg/kg) increased MF in liver (6.2 x control), kidney (2.4 x control), and lung (2.1 x control). These are known target organs for DMN carcinogenesis. No MF increase was observed in nontarget organs studied, i.e., bone marrow, bladder, and testis. Single ip treatment with DMN also increased lacI MF in liver but the increases were smaller than in a 5-daily-treatment regimen. This result suggests that multiple dosing is more effective in the transgenic mutation assay. The enhancement of cell proliferation observed was in bronchial epithelia 7 days after treatment. No micronucleus induction in peripheral blood was observed 24 hours after 2 and 3 daily ip treatments with 1 mg/kg DMN. An increase in the i...Continue Reading

References

Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S W KohlerJ M Short
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A GossenJ Vijg
Jan 1, 1995·Annual Review of Pharmacology and Toxicology·J C MirsalisR A Winegar
Jan 1, 1995·Environmental and Molecular Mutagenesis·N J Gorelick
Nov 10, 1994·Nature·T H Rabbitts
May 1, 1993·Mutagenesis·J C MirsalisJ M Short
Sep 1, 1995·Human & Experimental Toxicology·A NishikawaM Takahashi

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Citations

May 31, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Daisuke HibiTakashi Umemura
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·O MinowaT Noda
Jul 15, 2015·Mutagenesis·Lya G Soeteman-HernándezWout Slob
May 18, 2019·Archives of Toxicology·Christina HartmannNicole Schupp
Jun 6, 2021·Environmental and Molecular Mutagenesis·George E JohnsonAndreas Zeller
Aug 6, 2005·Mutation Research·Iain B LambertGeorge R Douglas

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