Organellar genome copy number variation and integrity during moderate maturation of roots and leaves of maize seedlings

Current Genetics
Jin Ma, Xiu-Qing Li

Abstract

Little information is available about organellar genome copy numbers and integrity in plant roots, although it was reported recently that the plastid and mitochondrial genomes were damaged under light, resulting in non-functional fragments in green seedling leaves in a maize line. In the present study, we investigated organellar genome copy numbers and integrity, after assessing the cellular ploidy, in seedling leaves and roots of two elite maize (Zea mays) cultivars using both long-fragment polymerase chain reaction (long-PCR) and real-time quantitative polymerase chain reaction (qPCR, a type of short-PCR). Since maize leaf and root cells are mainly diploid according to chromosome number counting and the literature, the DNA amount ratio between the organellar genomes and the nuclear genome could be used to estimate average organellar genome copy numbers per cell. In the present study, both long-PCR and qPCR analyses found that green leaves had dramatically more plastid DNA and less mitochondrial DNA than roots had in both cultivars. The similarity in results from long-PCR and qPCR suggests that green leaves and roots during moderate maturation have largely intact plastid and mitochondrial genomes. The high resolution of qPCR l...Continue Reading

References

Nov 1, 1996·The Plant Cell·X. Q. LiG. G. Brown
Nov 26, 2005·Molecular Genetics and Genomics : MGG·Weimin LiRalph Bock
Sep 15, 2007·Nature Protocols·Jaroslav DolezelJan Suda
Dec 1, 2007·Plant, Cell & Environment·Georgina PonceGladys I Cassab
Dec 15, 2010·The Plant Journal : for Cell and Molecular Biology·Tobias PreutenThomas Börner
May 24, 2011·Plant Science : an International Journal of Experimental Plant Biology·Wen-Huei ChenYu-Lin Kao
Dec 12, 2012·Planta·Delene J OldenburgArnold J Bendich
Mar 1, 2012·Wiley Interdisciplinary Reviews. Developmental Biology·Allison K StrohmPatrick H Masson
Jul 12, 2013·Current Genetics·Kaarel KrjutškovAndres Salumets
Jan 1, 1984·Planta·N S ScottJ V Possingham
Mar 29, 2014·The Plant Cell·Hieronim GolczykReinhold G Herrmann

❮ Previous
Next ❯

Citations

Apr 6, 2017·Molecular Biology and Evolution·Marc KrasovecGwenael Piganeau
Nov 6, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Suyan NiuXiu-Qing Li
Feb 23, 2019·Plant Molecular Biology·Kanagesswari MuniandySadequr Rahman
Dec 20, 2019·The Plant Journal : for Cell and Molecular Biology·Stephan GreinerReinhold G Herrmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.