Organic acid excretion by Streptomyces lividans TK24 during growth on defined carbon and nitrogen sources

Microbiology
T MaddenA P Ison

Abstract

Cultures of Streptomyces lividans TK24 grown in defined media containing certain rapidly used carbon and nitrogen sources excreted high levels of organic acids. These were identified by HPLC and enzymic assays as pyruvic acid and 2-oxoglutaric acid. Acidification occurred only with glucose as the principal carbon source, and depended on the nitrogen source used. With nitrate as the sole nitrogen source, high levels of pyruvate and small amounts of 2-oxoglutarate were produced. Carbon from D[U-14C]glucose was converted into both organic acids. Combining glucose with a selection of amino acids as primary nitrogen/secondary carbon sources yielded less pyruvate and more 2-oxoglutarate. Carbon from both 14C-labelled glucose and amino acids was metabolized to both organic acids. Adding nitrate to this combination caused a reversion of the acid production pattern to that of the glucose-nitrate combination, as if the amino acids were absent. Addition of ammonium salts to any combination of carbon and nitrogen sources completely prevented organic acid formation.

Citations

Oct 20, 2012·Applied Microbiology and Biotechnology·Peter KutasJan Kormanec
Sep 12, 2002·Journal of Microbiological Methods·Merja SuutariAino Nevalainen
Dec 11, 2007·Journal of Bacteriology·Nadria D GordonAmy M Gehring
Aug 28, 2010·Journal of Biotechnology·Pieter-Jan D'HuysJan F M Van Impe
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Oct 10, 2015·Microbial Cell Factories·Howbeer MuhamadaliRoyston Goodacre
Aug 10, 2000·Metabolic Engineering·F Naeimpoor, F Mavituna

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