Organic and inorganic selenium compounds inhibit mouse mammary cell growth in vitro by different cellular pathways

Cancer Letters
R SinhaD Medina

Abstract

Selenium, both organic and inorganic forms, inhibit mammary tumorigenesis in vivo and mammary cell growth in vitro. In the present study, sodium selenite was compared to methylselenocysteine (MSC) for their individual effects on cell growth, cdc2/cdk2 kinase activities and the levels of cyclins D1, E and A bound to cdk2 in a mouse mammary epithelial cell culture model. Selenite arrested the growth of cells in S-G2-M phase in contrast to MSC which arrested or delayed the cells in G1. In MSC-treated cells there was a 57% drop in the cdk2 kinase activity accompanied by a 73.5% decrease in cyclin E-cdk2 content as compared to the control cells. Selenite treatment increased the cdk2 kinase activity by 30% without any appreciable change in either of the cyclins D1, E or A bound to cdk2 when compared to the control cells. These data support the hypothesis that selenite and MSC have distinct modes of action in the inhibition of cell growth in vitro. Selenite has a strong genotoxic effect on the tumor cells; in contrast, MSC appears to inhibit cell growth via specific inhibition of cell cycle regulatory proteins.

References

Nov 1, 1992·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·C C ConawayK el-Bayoumy
Jul 1, 1992·Carcinogenesis·C Ip, H E Ganther
Feb 1, 1994·In Vitro Cellular & Developmental Biology. Animal·D J JerryJ S Butel
Apr 29, 1994·Biochemical Pharmacology·J LuH J Thompson
Jan 1, 1993·Molecular Biology of the Cell·L Connell-CrowleyJ W Harper

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Citations

Sep 28, 2010·Archives of Toxicology·Jela BrozmanováMiroslav Chovanec
Jan 13, 2010·Cancer Chemotherapy and Pharmacology·Sreenivasulu ChintalaYoucef M Rustum
Oct 28, 2008·Biological Trace Element Research·Inas A AsfourOmar M Fathey
Jan 25, 2006·Chemico-biological Interactions·M ValkoM Mazur
Mar 17, 2004·The Journal of Nutritional Biochemistry·Huawei Zeng, James H Botnen
Apr 11, 2001·Mutation Research·K El-Bayoumy
Oct 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Young R SeoMartin L Smith
Dec 12, 2002·Molecular Biology of the Cell·Liliana StalevaSeth J Orlow
Oct 17, 2006·Molecular Cancer Therapeutics·Rami G AzrakYoucef M Rustum
Dec 27, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Kiritkumar K VekariyaKulbhushan Tikoo
Aug 29, 2006·Toxicology·Lucia LetavayováJela Brozmanová
Aug 23, 2005·Toxicology Letters·Youn Wook ChungIck Young Kim
Nov 12, 2009·International Journal of Cancer. Journal International Du Cancer·Wenfeng FangWancai Yang
Apr 26, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P ErkekoğluF Hıncal
May 6, 2008·Free Radical Biology & Medicine·Numsen HailJulian E Spallholz
Mar 28, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Huawei Zeng
May 29, 2002·Oncogene·Young R SeoMartin L Smith
May 10, 2017·Biological Trace Element Research·Jing FangJunliang Deng
May 3, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Laura BanduraWlodzimierz Korohoda
Nov 10, 1998·The Journal of Nutrition·C Ip
Nov 9, 2016·Mini Reviews in Medicinal Chemistry·Boguslaw Lipinski
Apr 2, 2002·The Journal of Nutrition·Huawei Zeng

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