Organic carbon utilization by obligately and facultatively autotrophic beggiatoa strains in homogeneous and gradient cultures.

Applied and Environmental Microbiology
K D Hagen, D C Nelson

Abstract

Marine Beggiatoa strains MS-81-6 and MS-81-1c are filamentous gliding bacteria that use hydrogen sulfide and thiosulfate as electron donors for chemolithotrophic energy generation. They are known to be capable of chemolithoautotrophic growth in sulfide gradient media; here we report the first successful bulk cultivation of these strains in a defined liquid medium. To investigate their nutritional versatilities, strains MS-81-6 and MS-81-1c were grown in sulfide-oxygen gradient media supplemented with single organic compounds. Respiration rates and biomass production relative to those of controls grown in unsupplemented sulfide-limited media were monitored to determine whether organic compounds were utilized as sources of energy and/or cell carbon. With cells grown in sulfide gradient and liquid media, we showed that strain MS-81-6 strongly regulates two enzymes, the tricarboxylic acid cycle enzyme 2-oxoglutarate dehydrogenase and the Calvin cycle enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase, in response to the presence of organic carbon (acetate) in the growth medium. In contrast, strain MS-81-1c lacked 2-oxoglutarate dehydrogenase activity and regulated ribulose-1,5-bisphosphate carboxylase/oxygenase activity only sl...Continue Reading

References

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Citations

Oct 31, 2002·Applied and Environmental Microbiology·Heide N Schulz, Dirk De Beer
Sep 30, 2006·Biotechnology and Bioengineering·Xiumin JuRichard Bentley
Mar 21, 2017·Applied and Environmental Microbiology·Yvonne A LipsewersHenricus T S Boschker
Jul 2, 1999·Applied and Environmental Microbiology·S OtteB B Jorgensen
Jul 16, 2008·Applied and Environmental Microbiology·Talia JewellDouglas C Nelson
Jun 12, 2013·The Journal of Eukaryotic Microbiology·Kathryn J CoyneStephen C Cary
Mar 14, 2007·Molecular Systems Biology·Sergey StolyarDavid A Stahl
Sep 4, 2007·Applied and Environmental Microbiology·Susanne HinckHenk M Jonkers
Nov 5, 1999·Applied and Environmental Microbiology·N D GrayI M Head
Mar 1, 1996·Applied and Environmental Microbiology·S C McHattonD C Nelson

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