Organic Electrochemical Transistors for the Detection of Cell Surface Glycans

ACS Applied Materials & Interfaces
Lizhen ChenFeng Yan

Abstract

Cell surface glycans play critical roles in diverse biological processes, such as cell-cell communication, immunity, infection, development, and differentiation. Their expressions are closely related to cancer growth and metastasis. This work demonstrates an organic electrochemical transistor (OECT)-based biosensor for the detection of glycan expression on living cancer cells. Herein, mannose on human breast cancer cells (MCF-7) as the target glycan model, poly dimethyl diallyl ammonium chloride-multiwall carbon nanotubes (PDDA-MWCNTs) as the loading interface, concanavalin A (Con A) with active mannose binding sites, aptamer and horseradish peroxidase co-immobilized gold nanoparticles (HRP-aptamer-Au NPs) as specific nanoprobes are used to fabricate the OECT biosensor. In this strategy, PDDA-MWCNT interfaces can enhance the loading of Con A, and the target cells can be captured through Con A via active mannose binding sites. Thus, the expression of cell surface can be reflected by the amount of cells captured on the gate. Specific nanoprobes are introduced to the captured cells to produce an OECT signal because of the reduction of hydrogen peroxide catalyzed by HRP conjugated on Au nanoparticles, while the aptamer on nanoprobe...Continue Reading

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Citations

Feb 1, 2020·Biomicrofluidics·Kevin D DorfmanC Daniel Frisbie
Nov 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ariana Villarroel MarquezAmir Pakdel
Mar 7, 2021·International Journal of Molecular Sciences·Margarita Vázquez-González, Itamar Willner
May 5, 2021·Advanced Biology·Sarah L BarronRóisín M Owens
Jan 9, 2019·Accounts of Chemical Research·Naixiang WangFeng Yan
Oct 14, 2021·ACS Applied Materials & Interfaces·Songyan Yu, Erin L Ratcliff
Dec 16, 2021·Chemical Reviews·Charalampos PitsalidisRóisín M Owens

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