Organic osmolytes in aerobic bacteria from mono lake, an alkaline, moderately hypersaline environment.

Applied and Environmental Microbiology
R A CiullaM F Roberts

Abstract

The identity and concentrations of intracellular organic solutes were determined by nuclear magnetic resonance spectroscopy for two strains of aerobic, gram-negative bacteria isolated from Mono Lake, Calif., an alkaline, moderately hypersaline lake. Ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) was the major endogenous solute in both organisms. Concentrations of ectoine varied with external NaCl levels in strain ML-D but not in strain ML-G, where the level was high but invariant from 1.5 to 3.0 M NaCl. Hydroxyectoine also occurred in strain ML-D, especially at elevated NaCl concentrations (2.5 and 3.0 M), but at levels lower than those of ectoine. Exogenous organic solutes that might occur in Mono Lake were examined for their effects on the de novo synthesis of ectoine. Dimethylsulfoniopropionate (DMSP) (0.1 or 1 mM) did not significantly lower ectoine levels in either isolate, and only strain ML-G showed any capacity for DMSP accumulation. With nitrogen limitation, however, DMSP (0.1 mM) substituted for ectoine in strain ML-G and became the main organic solute. Glycine betaine (GB) was more effective than DMSP in affecting ectoine levels, principally in strain ML-D. Strain ML-D accumulated GB to 50 or 67% o...Continue Reading

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Citations

Nov 9, 2016·Critical Reviews in Microbiology·Susan B Watson, Friedrich Jüttner
Apr 1, 2004·FEMS Microbiology Ecology·Ronald S OremlandJames T Hollibaugh
Nov 28, 2012·The ISME Journal·Chakkiath Paul AntonyYogesh S Shouche
Sep 8, 2006·Applied Microbiology and Biotechnology·Georg Lentzen, Thomas Schwarz
Jul 5, 2016·Frontiers in Microbiology·Zachary T AanderudAlan R Harker
Aug 1, 2020·Frontiers in Microbiology·Akhilesh KumarJay Prakash Verma
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·A VentosaA Oren
May 1, 1997·Journal of Bacteriology·D DesmaraisM F Roberts

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