Organic solvent-tolerant bacterium which secretes an organic solvent-stable proteolytic enzyme.

Applied and Environmental Microbiology
H OginoH Ishikawa

Abstract

A bacterial strain which can be grown in a medium containing organic solvents and can secrete a proteolytic enzyme was isolated and identified as Pseudomonas aeruginosa. The strain was derived by the following two-step procedures: high proteolytic enzyme producers were first isolated by the usual method, and then the organic solvent-tolerant microorganism was selected from these high-rate proteolytic enzyme producers. The proteolytic activity of the supernatant of the culture was stable in the presence of various organic solvents. The stability of the enzyme in the presence of organic solvents, of which the values of the logarithm of the partition coefficient (log P) were equal to or more than 3.2, was almost the same as that in the absence of organic solvents. It is expected that both the solvent-tolerant microorganism and the solvent-stable enzyme produced by this strain can be used as catalysts for reactions in the presence of organic solvents.

References

Oct 1, 1994·Applied and Environmental Microbiology·H OginoH Ishikawa
Jul 1, 1987·Biotechnology and Bioengineering·C LaaneC Veeger

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Citations

Apr 23, 2008·Applied Microbiology and Biotechnology·Ritu Sareen, Prashant Mishra
Jan 15, 2016·Biological Procedures Online·Ashok KumarPankaj Kumar Arora
Jan 20, 2010·Protein Engineering, Design & Selection : PEDS·Hiroyasu OginoNoriyuki Doukyu
May 12, 2007·Journal of Industrial Microbiology & Biotechnology·Raja Noor Zaliha Raja Abd RahmanMahiran Basri
Jan 19, 2010·Journal of Basic Microbiology·Raja Noor Zaliha Raja Abd RahmanAbu Bakar Salleh
Feb 24, 2010·Applied Biochemistry and Biotechnology·Sasithorn UttatreeJittima Charoenpanich
Jun 12, 2009·Critical Reviews in Biotechnology·Anshu Gupta, S K Khare
Mar 24, 2016·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·P Anbu
Apr 15, 2004·Biotechnology and Bioengineering·Iulian M BobeHaruo Ishikawa
Aug 22, 2009·Journal of Industrial Microbiology & Biotechnology·Tony Marcio da SilvaMaria Lourdes T M de Polizeli
Oct 23, 2008·Journal of Industrial Microbiology & Biotechnology·Jignasha T Thumar, Satya P Singh
May 29, 2010·The Protein Journal·Tengku Haziyamin Tengku Abdul HamidMahiran Basri
Jan 31, 2007·Applied Microbiology and Biotechnology·Hermann J HeipieperFriedhelm Meinhardt
Oct 22, 2011·International Journal of Molecular Sciences·Raja Noor Zaliha Raja Abd RahmanChee Fah Wong
Apr 6, 2021·Open Life Sciences·Mihaela Marilena Stancu
Jul 1, 1997·Journal of Bacteriology·H C Pinkart, D C White
May 25, 2007·Biochemical and Biophysical Research Communications·Hiroyasu OginoHaruo Ishikawa
Dec 12, 2002·Chemical Reviews·Frank Bordusa

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