DOI: 10.1101/509992Jan 2, 2019Paper

Organism-wide single-cell transcriptomics of long-lived C. elegans daf-2 mutants reveals tissue-specific reprogramming of gene expression networks

BioRxiv : the Preprint Server for Biology
Jessica PrestonMaggie Weitzman

Abstract

A critical requirement for a systems-level understanding of complex biological processes such as aging is the ability to directly characterize interactions between cells and tissues within a multicellular organism. C. elegans nematodes harboring mutations in the insulin-like receptor daf-2 exhibit dramatically-increased lifespans. To identify tissue-specific biochemical mechanisms regulating aging plasticity, we single-cell sequenced mRNA libraries generated from seven populations of whole day one adult wild-type and daf-2 worms using the 10x ChromiumV1 platform. The age-synchronized samples were bioinformatically merged into a single aligned dataset containing 40,000 age-synchronized wild-type and daf-2 cellular transcriptomes partitioned into 101 clusters, using unsupervised machine-learning algorithms to identify common cell types. Here we describe the basic features of the adult C. elegans single-cell transcriptome and summarize functional alterations observed in the gene expression profiles of long-lived daf-2 worms. Comprehensive methods and datasets are provided. This is the first study to directly quantify cell-specific differential gene expression between two age synchronized, genetically distinct populations of multic...Continue Reading

Related Concepts

Aging
Gene Clusters
Gene Expression
Learning
Neuronal Plasticity
Single cell proteins
Antigens, CD55
Insulin-Like Growth Factor Receptor
Caenorhabditis elegans
RNA library

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