PMID: 3759909Oct 1, 1986Paper

Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies

Journal of Bacteriology
J A KanabrockiF R Pond

Abstract

Type 51 R bodies are produced by all bacterial endosymbionts (Caedibacter taeniospiralis) of Paramecium tetraurelia that confer the hump-killer trait upon their hosts. Type 51 R-body synthesis by C. taeniospiralis is required for expression of the hump-killer trait. The genetic determinants for type 51 R-body synthesis by C. taeniospiralis 47 have been cloned and expressed in Escherichia coli. In this communication we describe three species of polypeptides required for R-body synthesis and the organization of their genetic determinants. Each polypeptide species is controlled by a separate gene that is expressed as an independent transcriptional unit possessing regulatory signals that are recognized by E. coli. Two polypeptide species of 10 and 18 kilodaltons are required for R-body synthesis but apparently are not structural subunits. The third polypeptide species (13 kilodaltons) is the major structural subunit. R-body assembly involves polymerization reactions that result in high-molecular-mass polypeptide complexes, primarily composed of the 13-kilodalton polypeptide species, that appear to be the result of covalent cross-linking between structural subunits. The results presented here have been suggested to apply to the asse...Continue Reading

References

Jun 1, 1979·Journal of Bacteriology·J C McMichael, J T Ou
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Apr 1, 1986·Journal of Bacteriology·R L QuackenbushB J Cox
Jun 1, 1974·Bacteriological Reviews·J R PreerA Jurand
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D B Clewell, D R Helinski
Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J R Preer, L B Preer
Oct 1, 1981·Journal of Bacteriology·Y EshdatN Sharon
Aug 1, 1984·Journal of Bacteriology·P E Orndorff, S Falkow
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R L Quackenbush, J A Burbach

❮ Previous
Next ❯

Citations

Mar 2, 2013·G3 : Genes - Genomes - Genetics·Kasie RaymannSimonetta Gribaldo
Jul 1, 2017·BMC Biology·James J RussellAnne Brunet
Mar 1, 1989·Microbiological Reviews·F R PondR L Quackenbush
Jan 28, 2016·ACS Synthetic Biology·Jessica K Polka, Pamela A Silver

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.