PMID: 37238Aug 1, 1979

Organization and transfer of heterologous chloramphenicol and tetracycline resistance genes in pneumococcus

Journal of Bacteriology
N B ShoemakerW R Guild

Abstract

The cat and tet genes of chloramphenicol- and tetracycline-resistant clinical isolates of Streptococcus pneumoniae from Paris and Japan were shown to be contained in adjacent heterologous insertions into the chromosome. The two insertions transformed laboratory strains at frequencies that were low, unequal, and, for tet, very sensitive to the length of the donor deoxyribonucleic acid strand. In contrast, the transforming activity of cat was relatively stable. There was an unusual asymmetric cotransfer, in that a majority of the tet transformants also acquired cat, whereas only a few of the cat transformants also acquired tet. The evidence for chromosomal insertion came from genetic data showing linkage of cat to a chromosomal gene and from cosedimentation of cat with chromosomal markers in both velocity and dye-buoyancy experiments. Genes on a known plasmid introduced into pneumococcus from Streptococcus faecalis showed very different physical behavior. Most of the transformation properties of these genes can be readily accounted for by analogy to transformation of deletions of normal genes. Whether transposition contributes any of the transfers remains to be determined. The presence of one of the genes in the recipient promote...Continue Reading

Citations

Nov 1, 1986·Molecular & General Genetics : MGG·P Courvalin, C Carlier
Mar 1, 1988·European Journal of Epidemiology·G Pozzi, W R Guild
Jul 17, 2004·Molecular Microbiology·Lauren J McAllisterJames C Paton
Jun 11, 2011·FEMS Microbiology Reviews·Adam P Roberts, Peter Mullany
Nov 20, 2014·Nature Communications·Nicholas J CroucherWilliam P Hanage
Jan 1, 1980·Molecular & General Genetics : MGG·C W Saunders, W R Guild
Jan 1, 1981·Molecular & General Genetics : MGG·C W Saunders, W R Guild
Nov 18, 1982·Nature·C Gawron-Burke, D B Clewell
May 20, 2004·Journal of Bacteriology·Krzysztof TrzcińskiMarc Lipsitch
Jan 15, 1996·FEMS Microbiology Letters·R ProvvediG Pozzi
Dec 4, 2013·Antimicrobial Agents and Chemotherapy·Francesco IannelliGianni Pozzi

Related Concepts

Syntomycin
Genome Mapping
Chromosomes, Bacterial
Antibiotic Resistance, Microbial
Cistron
Streptococcus pneumoniae
Topicycline
Transformation, Bacterial

Related Feeds

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.