PMID: 1202021Dec 1, 1975Paper

Organization of an actin filament-membrane complex. Filament polarity and membrane attachment in the microvilli of intestinal epithelial cells

The Journal of Cell Biology
M S Mooseker, L G Tilney

Abstract

The association of actin filaments with membranes is now recognized as an important parameter in the motility of nonmuscle cells. We have investigated the organization of one of the most extensive and highly ordered actin filament-membrane complexes in nature, the brush border of intestinal epithelial cells. Through the analysis of isolated, demembranated brush borders decorated with the myosin subfragment, S1, we have determined that all the microvillar actin filaments have the same polarity. The S1 arrowhead complexes point away from the site of attachment of actin filaments at the apical tip of the microvillar membrane. In addition to the end-on attachment of actin filaments at the tip of the microvillus, these filaments are also connected to the plasma membrane all along their lengths by periodic (33 nm) cross bridges. These bridges were best observed in isolated brush borders incubated in high concentrations of Mg++. Their visibility is attributed to the induction of actin paracrystals in the filament bundles of the microvilli. Finally, we present evidence for the presence of myosinlike filaments in the terminal web region of the brush border. A model for the functional organization of actin and myosin in the brush border ...Continue Reading

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Citations

Jul 25, 1998·Cell Motility and the Cytoskeleton·U WolfrumD S Williams
Sep 1, 1977·The American Journal of Anatomy·N KrishnanM Singer
Jul 1, 1978·The American Journal of Anatomy·R A Jersild, R W Crawford
Jan 1, 1976·Journal of Supramolecular Structure·R D GoldmanJ A Schloss
Jan 1, 1976·Journal of Supramolecular Structure·T D Pollard
Jan 1, 1978·Journal of Supramolecular Structure·S J SingerD Louvard
Jan 1, 1979·Journal of Supramolecular Structure·L A CulpB J Rollins
Jan 1, 1979·Journal of Supramolecular Structure·P R Stewart, J A Spudich
Jan 1, 1979·Journal of Supramolecular Structure·T J Bradley, P Satir
Jan 1, 1982·Cell and Tissue Research·N Slepecky, S C Chamberlain
Jan 1, 1982·Archives of Oto-rhino-laryngology·N Slepecky, S C Chamberlain
Jan 1, 1981·Archives of Oto-rhino-laryngology·H P Zenner
Jan 29, 2010·Cellular and Molecular Life Sciences : CMLS·Rajalakshmi NambiarMatthew J Tyska
Jan 1, 1984·Comparative Biochemistry and Physiology. A, Comparative Physiology·M W SmithM A Peacock
Jan 1, 1983·Molecular and Cellular Endocrinology·B M Jockusch
Jan 1, 1981·Neuroscience·R P Rees, T S Reese

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