Organization of descending neurons in Drosophila melanogaster

Scientific Reports
Cynthia T Hsu, Vikas Bhandawat

Abstract

Neural processing in the brain controls behavior through descending neurons (DNs) - neurons which carry signals from the brain to the spinal cord (or thoracic ganglia in insects). Because DNs arise from multiple circuits in the brain, the numerical simplicity and availability of genetic tools make Drosophila a tractable model for understanding descending motor control. As a first step towards a comprehensive study of descending motor control, here we estimate the number and distribution of DNs in the Drosophila brain. We labeled DNs by backfilling them with dextran dye applied to the neck connective and estimated that there are ~1100 DNs distributed in 6 clusters in Drosophila. To assess the distribution of DNs by neurotransmitters, we labeled DNs in flies in which neurons expressing the major neurotransmitters were also labeled. We found DNs belonging to every neurotransmitter class we tested: acetylcholine, GABA, glutamate, serotonin, dopamine and octopamine. Both the major excitatory neurotransmitter (acetylcholine) and the major inhibitory neurotransmitter (GABA) are employed equally; this stands in contrast to vertebrate DNs which are predominantly excitatory. By comparing the distribution of DNs in Drosophila to those rep...Continue Reading

References

Nov 1, 1977·Journal of the Neurological Sciences·B E Tomlinson, D Irving
Sep 1, 1992·Comparative Biochemistry and Physiology. Comparative Physiology·J Kien, J S Altman
Mar 1, 1991·Neurochemical Research·D B SattelleJ J Rauh
Oct 1, 1991·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·W Gronenberg, N J Strausfeld
Nov 1, 1988·The Journal of Comparative Neurology·R J Nudo, R B Masterton
Feb 13, 1995·The Journal of Comparative Neurology·M Mizunami
Nov 22, 1994·The Journal of Comparative Neurology·K P Rajashekhar, R N Singh
Nov 13, 1995·The Journal of Comparative Neurology·M Mizunami
Jan 7, 1998·Trends in Neurosciences·A M HosieR H ffrench-Constant
May 20, 1999·Microscopy Research and Technique·K Yasuyama, P M Salvaterra
Apr 25, 2001·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·E M Staudacher
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·C C Labandeira, T L Phillips
Dec 20, 2003·Science·Rachel I WilsonGilles Laurent
Mar 17, 2004·Brain Research. Gene Expression Patterns·P M Salvaterra, T Kitamoto
Apr 12, 2005·Brain, Behavior and Evolution·Stefanie MaresWulfila Gronenberg
Oct 7, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rachel I Wilson, Gilles Laurent
Jun 28, 2006·The Journal of Comparative Neurology·Hideo Otsuna, Kei Ito
Nov 14, 2006·The Journal of Comparative Neurology·Nicholas J Strausfeld, Jun-Ya Okamura
Feb 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Juergen HaagAlexander Borst
Mar 21, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Adrian WertzJuergen Haag
Jun 19, 2008·Annual Review of Neuroscience·Roger N Lemon
Feb 17, 2009·Neuron·Michael D Gordon, Kristin Scott
Feb 24, 2009·The Journal of Comparative Neurology·Sebastian BuschHiromu Tanimoto
Mar 13, 2009·Nature·Azusa KamikouchiKei Ito
Apr 7, 2009·Bioinformatics·Stephan PreibischPavel Tomancak
May 29, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Myungin Baek, Richard S Mann
Jun 26, 2009·BMC Biology·Brian M WiegmannDavid K Yeates
Nov 20, 2009·Current Biology : CB·Lars Chittka, Jeremy Niven
Nov 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Adrian WertzAlexander Borst
May 4, 2010·Neuron·Shawn R OlsenRachel I Wilson
Jun 8, 2010·Nature Methods·Johannes D SeeligVivek Jayaraman
Sep 10, 2010·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·John R GrayR Meldrum Robertson

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Citations

Jul 23, 2016·Frontiers in Physiology·Shigehiro Namiki, Ryohei Kanzaki
Apr 11, 2017·Current Biology : CB·Bettina SchnellMichael H Dickinson
Jul 15, 2017·Cell·Alice A RobieKristin Branson
Jun 27, 2018·ELife·Jessica CandeGordon J Berman
Aug 6, 2018·Cell and Tissue Research·Shigehiro Namiki, Ryohei Kanzaki
Jun 27, 2018·ELife·Matthieu Louis, Julie H Simpson
Jan 29, 2020·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Sarah NicholasKarin Nordström
Mar 11, 2020·Frontiers in Physiology·Stav EmanuelFrederic Libersat
Sep 14, 2018·Nature Communications·Ricardo ZacariasMarta A Moita
Apr 6, 2018·Genetics·Julie H Simpson, Loren L Looger
May 11, 2018·Cell and Tissue Research·Joss von HadelnUwe Homberg
Jul 10, 2019·Annual Review of Neuroscience·Ludwig Ruder, Silvia Arber
Nov 4, 2020·ELife·Catherine E SchretterGerald M Rubin
Mar 24, 2020·Cell·Alexandros K KanellopoulosClaudia Bagni
May 26, 2021·ELife·Philipp SchlegelGregory S X E Jefferis
Sep 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Sarah Nicholas, Karin Nordström

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Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

ImageJ

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